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| Product Name | Lambda Exonuclease (5 U/uL) |
| Catalog No. | IVDRM-0495 |
| Description | Derived from bacteriophage lambda, this exonuclease acts on double stranded DNA and removes 5 prime mononucleotides processively in the 5 prime to 3 prime direction. It recognises and selectively digests duplex DNA that carries a 5 prime phosphate, while single stranded DNA and DNA lacking a 5 prime phosphate are degraded only very slowly. This selectivity preserves the complementary strand and makes it straightforward to prepare high purity single stranded DNA, and it also suits pre-treatment of PCR products before cloning. Supplied at 5 U/uL, the preparation is at least 95% pure, holds endotoxin below 10 EU per mg and has very low nickase and RNase residue. |
| Intended Use | Preparation of high purity single stranded DNA and pre-treatment of PCR products before cloning |
| Principle / Technology | Processive 5 prime to 3 prime exonuclease digestion specific for 5 prime phosphorylated double stranded DNA |
| Sample Type | 5 prime phosphorylated double stranded DNA; PCR products |
| Specificity | Selective for 5 prime phosphorylated duplex DNA; very slow on single stranded or unphosphorylated DNA |
| Reaction Conditions / Protocol | Incubate with 5 prime phosphorylated duplex DNA to degrade the phosphorylated strand and retain the complementary strand |
| Storage Conditions | -25 to -15°C |
| Shelf Life | 2 years |
| Package Specifications | 1000 U; 5000 U |
| Quality Control | Endotoxin below 10 EU per mg; very low nickase and RNase residue |
| Key Features | Preserves the complementary strand for single stranded DNA preparation; low endotoxin and residue levels |
| Purity | Greater than or equal to 95% |
| Concentration | 5 U/uL |
| Readouts / Downstream Assays | Agarose gel electrophoresis of single stranded DNA product |
For in vitro diagnostic use.
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