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| Product Name | L-Glutamine, 200 mM Solution, 100x |
| Catalog No. | ATR-CCR-0010 |
| Description | A 100-fold concentrated sterile L-glutamine solution at 200 mM in 0.85% NaCl, for supplementation of cell culture media to replace glutamine degraded during storage or to meet elevated metabolic demands. |
| Intended Use | Supplement of cell culture media with labile L-glutamine to the standard 2 mM working concentration; addition to media lacking glutamine or for supplementing glutamine in high-density cultures. |
| Principle / Technology | L-glutamine is an essential amino acid for most cultured cells, serving as a major energy source and nitrogen donor for purine and pyrimidine synthesis; it spontaneously degrades in solution (half-life ~3 weeks at 4°C), necessitating fresh supplementation. |
| Detection Method | Cell growth and viability comparison with and without glutamine supplementation, ammonia concentration monitoring |
| Sample Type | All mammalian cell culture media types |
| Performance Range / Specifications | 200 mM concentrate provides 2 mM working concentration at 1:100 dilution |
| Sensitivity / LOD | N/A |
| Specificity | N/A |
| Reaction Conditions / Protocol | Add 5 mL per 500 mL medium (1:100 dilution); replace medium every 3–4 days or supplement additional glutamine for extended culture between medium changes. |
| Components / Formulation | L-Glutamine 200 mM (29.2 mg/mL) in 0.85% sodium chloride, sterile-filtered |
| Storage Conditions | -20°C (long term); 2–8°C after thawing; aliquot to avoid repeated freeze-thaw |
| Shelf Life | 24 months at -20°C; 3 months at 2–8°C |
| Package Specifications | 100 mL, 500 mL |
| Product Form | Liquid concentrate (100x), sterile |
| Quality Control | Concentration verification, sterility, endotoxin <0.25 EU/mL, cell growth promotion |
| Key Features | Sterile 100x concentrate; ready to use; essential for glutamine-free media; aliquot-friendly packaging; validated for all mammalian cells |
For research use only, not for clinical use.
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