KpnI Rapid Restriction Endonuclease, High Concentration (100 U/uL)
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KpnI Rapid Restriction Endonuclease, High Concentration (100 U/uL)

Cat.No: IVDRM-0433 Datasheet

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Product Details Related Products
Product Name KpnI Rapid Restriction Endonuclease, High Concentration (100 U/uL)
Catalog No. IVDRM-0433
Description Recognising 5 prime GGTAC^C 3 prime, this high concentration rapid recombinant endonuclease rapidly cuts plasmid DNA, PCR products and genomic DNA within 5 to 15 minutes. Supplied at 100 U/uL, it operates in the shared rapid series buffer at 37°C and is heat inactivated at 80°C for 20 minutes. Dam, Dcm, CpG, EcoKI and EcoBI methylation all leave activity unchanged, giving predictable digestion across commonly used template sources, and no alternative named isoschizomers are documented.
Intended Use Rapid DNA cutting for molecular cloning workflows
Principle / Technology Site specific cleavage at 5 prime GGTAC^C 3 prime within 5 to 15 minutes at 37°C
Specificity Unaffected by Dam, Dcm, CpG, EcoKI and EcoBI methylation; no alternative named isoschizomers
Reaction Conditions / Protocol Digest in 1x rapid restriction buffer at 37°C for 5 to 15 minutes; heat inactivate at 80°C for 20 minutes
Storage Conditions -25 to -15°C
Shelf Life 2 years
Package Specifications 200 T
Key Features High concentration 100 U/uL format; complete methylation tolerance across common systems
Concentration 100 U/uL
Readouts / Downstream Assays Restriction digest pattern on agarose gel

For in vitro diagnostic use.

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