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| Product Name | Klenow Fragment, 3′→5′ Exonuclease-Deficient Mutant |
| Catalog No. | IVDRM-0334 |
| Description | This mutant retains the 5′ to 3′ polymerase activity of the E. coli DNA polymerase I large fragment while engineered mutations remove both the 5′ to 3′ and the 3′ to 5′ exonuclease activities. Without exonucleolytic degradation of primers or templates, it gives cleaner performance in random-primer labelling, dideoxy sequencing and NGS library workflows. |
| Intended Use | Random-primer DNA labelling; dideoxy DNA sequencing; NGS library construction. |
| Storage Conditions | -25 °C to -15 °C |
| Shelf Life | 24 months |
| Package Specifications | 200 U, 1000 U and 5000 U packs |
| Activity / Unit Definition | 5′→3′ DNA polymerase activity retained; both 5′→3′ and 3′→5′ exonuclease activities removed by mutation |
| Source / Origin | Engineered proteolytic fragment of E. coli DNA polymerase I |
| Model Type | Exonuclease-deficient DNA polymerase I large fragment |
For in vitro diagnostic use.
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