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| Product Name | IMDM (Iscove's Modified Dulbecco's Medium), with 25 mM HEPES |
| Catalog No. | ATR-CCR-0007 |
| Description | A highly enriched modification of DMEM containing selenium, additional amino acids, vitamins, and HEPES buffer, specifically formulated for the high-density culture of hematopoietic cells and B lymphocyte hybridomas. |
| Intended Use | High-density suspension culture of hybridoma cells for monoclonal antibody production; growth of B and T lymphocytes, macrophages, dendritic cells, and hematopoietic progenitor cells; culture of cells in serum-free conditions. |
| Principle / Technology | Further enrichment of DMEM with potassium nitrate (replacing ferric nitrate), additional amino acids (asparagine, serine), selenium, and HEPES buffer supports cell densities exceeding 10^7 cells/mL for high-producing hybridomas. |
| Detection Method | IgG production by ELISA from hybridoma culture supernatant, flow cytometric analysis of cell surface markers |
| Sample Type | Hybridoma cell lines, lymphocytes, macrophages, dendritic cells, bone marrow cells, hematopoietic stem cells |
| Performance Range / Specifications | Supports hybridoma cell densities up to 10^7 cells/mL; monoclonal antibody yields 100–500 mg/L |
| Sensitivity / LOD | N/A |
| Specificity | N/A |
| Reaction Conditions / Protocol | Supplement with 10–20% heat-inactivated FBS, 50 µM 2-mercaptoethanol, and 1% penicillin-streptomycin; equilibrate in 5% CO2; culture in suspension with gentle agitation or stationary. |
| Components / Formulation | IMDM base with 4.5 g/L glucose, 25 mM HEPES, sodium bicarbonate, selenium, additional amino acids, 2 mM L-glutamine, phenol red |
| Storage Conditions | 2–8°C, protect from light |
| Shelf Life | 12 months |
| Package Specifications | 500 mL, 1 L |
| Product Form | Liquid, sterile-filtered |
| Quality Control | Sterility, endotoxin <0.25 EU/mL, pH, osmolality, hybridoma growth and antibody production validation |
| Key Features | Enriched formulation for lymphoid cells; high-density culture capable; selenium supplemented; HEPES buffered; standard for hybridoma production |
For research use only, not for clinical use.
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