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| Product Name | Human Peripheral Blood Lymphocyte Separation Medium, Sterile, BioReagent Grade |
| Catalog No. | RERE-0235 |
| Description | Sterile, low-endotoxin density gradient medium for isolating lymphocytes from human peripheral blood or cord blood. Separation follows the density differences among blood cell types, with erythrocytes and granulocytes near 1.090 g/mL, lymphocytes and monocytes between 1.075 and 1.090 g/mL, and platelets at 1.030 to 1.035 g/mL, so centrifugation arranges the cells into distinct layers. The mononuclear band is then withdrawn for culture or further analysis. Supplied as a liquid for storage at room temperature. |
| Intended Use | Density gradient isolation of lymphocytes and mononuclear cells from human blood or cord blood |
| Principle / Technology | Buoyant density differences among blood cell populations drive layer formation during centrifugation |
| Specificity | Sterile filtered; BioReagent grade; endotoxin tested; for cell culture; erythrocytes and granulocytes near 1.090 g/mL, lymphocytes and monocytes 1.075-1.090 g/mL, platelets 1.030-1.035 g/mL; liquid; 200 mL |
| Reaction Conditions / Protocol | Dilute anticoagulated whole blood with an equal volume of PBS or saline, layer it over the medium at a 2 to 1 to 1 ratio with the sample and isotonic solution, centrifuge at 1000 x g for 30-40 min, collect the mononuclear band and wash at 300 x g for 10 min; avoid buffers that contain calcium or magnesium |
| Storage Conditions | Room temperature |
| Package Specifications | 200 mL |
| Key Features | Sterile and low endotoxin; cell culture tested; explicit density data supplied |
For research use only, not for clinical use.
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