Human Peripheral Blood Lymphocyte Separation Medium, FICOLL-Based
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Human Peripheral Blood Lymphocyte Separation Medium, FICOLL-Based

Cat.No: RERE-0230 Datasheet

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Product Name Human Peripheral Blood Lymphocyte Separation Medium, FICOLL-Based
Catalog No. RERE-0230
Description Density-gradient separation medium prepared from FICOLL for isolating peripheral blood lymphocytes from human blood samples, also listed as a sample density separation medium. Layered under diluted blood and centrifuged, the medium partitions the sample so lymphocytes band at the interface for harvest while erythrocytes and granulocytes sediment through. The recovered population feeds human immunology workflows including flow cytometry, proliferation assays and culture setup. Unopened stock keeps for 24 months at room temperature protected from light.
Intended Use Density-gradient isolation of peripheral blood lymphocytes from human blood for immunology workflows
Principle / Technology FICOLL-based density gradient partitions human blood during centrifugation so lymphocytes band at the interface
Specificity FICOLL-based density-gradient medium; 200 mL format; room temperature storage protected from light; 24 month shelf life
Reaction Conditions / Protocol Dilute the blood sample, layer over the medium, centrifuge under the protocol conditions and harvest the lymphocyte interface, then wash before downstream use
Storage Conditions RT, protect from light
Package Specifications 200 mL
Key Features FICOLL-based formulation; human PB lymphocyte format; 24 month shelf life

For research use only, not for clinical use.

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