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| Product Name | Human Intestinal Cancer Organoid Growth Medium Plus |
| Catalog No. | RESK-0698 |
| Description | Human Intestinal Cancer Organoid Growth Medium Plus is a serum-free complete medium designed for the establishment and long-term culture of intestinal cancer organoids derived from human cells or tissues. In the presence of a basement membrane matrix, the medium's specific components and abundant cytokines promote the rapid growth of intestinal cancer cells and their efficient formation into intestinal cancer organoids. The organoid formation process is smooth and rapid while maintaining high intestinal cancer cell identity and viability, supporting downstream studies of physiological function, disease research, and precision medicine. This Plus version is an upgraded formulation of the standard Human Intestinal Cancer Organoid Growth Medium. The buffer system and nutrient composition have been optimized, improving the medium's resistance to external environmental stress and its overall stability. |
| Intended Use | For the establishment and long-term culture of human intestinal cancer organoids from cell- or tissue-derived samples. Applications include tumor disease modeling, drug efficacy evaluation, toxicity assessment, and precision medicine research. |
| Principle / Technology | Organoid culture technology. When cells or tissue fragments are embedded in a basement membrane matrix, the medium's specific components and abundant cytokines drive intestinal cancer cells to rapidly grow and self-organize into three-dimensional intestinal cancer organoids while retaining tumor heterogeneity. |
| Sample Type | Cells, tissues |
| Performance Range / Specifications | Broad applicability: suitable for tumor disease modeling, drug efficacy evaluation, and toxicity assessment. Efficient and stable culture performance while retaining tumor heterogeneity. Plus version with optimized buffer system and nutrient composition for improved resistance to environmental stress and enhanced stability. |
| Reaction Conditions / Protocol | Embed isolated human intestinal cancer cells or tissue fragments in a basement membrane matrix, then overlay with this medium for organoid establishment and long-term culture. Thaw the medium at 2 °C to 8 °C until completely melted before use. |
| Components / Formulation | Serum-free complete medium containing specific proprietary components and abundant cytokines optimized for human intestinal cancer organoid growth; upgraded Plus formulation with an optimized buffer system and nutrient composition. |
| Storage Conditions | Store frozen at -20 °C or below. Avoid repeated freeze-thaw cycles. Thaw at 2 °C to 8 °C until completely melted before use. If the medium will not be used up within a short period (3 months), aliquot for storage to avoid repeated freeze-thaw. |
| Shelf Life | 12 months when stored at -20 °C or below. |
| Package Specifications | 100 mL, 500 mL |
| Product Form | Liquid |
| Expiration Date / Stability | Stable for 12 months at -20 °C or below. Avoid repeated freeze-thaw cycles; aliquot if the medium will not be consumed within 3 months. |
| Regulatory / Compliance | For research use only. |
| Compatibility | Suitable for culturing organoids from both human small intestine and colon. Used together with a basement membrane matrix for three-dimensional organoid culture. |
| Recommended Buffer System | Optimized buffer system in the Plus formulation, providing improved resistance to external environmental stress and enhanced medium stability. |
| Application Notes / Precautions | Applicable to intestinal cancer organoids derived from human cells or tissues; suitable for both small intestine and colon organoids. Supports tumor disease modeling, drug efficacy evaluation, toxicity assessment, and precision medicine research. Thaw at 2 °C to 8 °C until completely melted before use. Avoid repeated freeze-thaw cycles; aliquot for storage if the medium will not be used up within 3 months. |
For research use only, not for clinical use.
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