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| Product Name | Human Intestinal Cancer Organoid Culture Kit |
| Catalog No. | RESK-0701 |
| Description | The Human Intestinal Cancer Organoid Culture Kit consists of two components: an organoid culture basement membrane matrix (phenol red-free, LDEV-free, EHS tumor-derived) and a human intestinal cancer organoid growth medium Plus. The matrix is a sterile preparation extracted from Engelbreth-Holm-Swarm (EHS) mouse tumors, mainly containing laminin, type IV collagen, heparan sulfate proteoglycan (HSPG), entactin (nidogen), and growth factors such as TGF-β and EGF. At room temperature it polymerizes to form a biologically active three-dimensional matrix that mimics the structure and function of the in vivo basement membrane. It has been validated for organoid culture and provides the 3D environmental support required for organoid construction. The human intestinal cancer organoid growth medium Plus is a serum-free formulation containing proprietary components and abundant cytokines. It enables intestinal cancer cells to grow rapidly and form organoids in an efficient and stable manner while maintaining good cellular characteristics and viability. This kit is used to efficiently establish and long-term culture human intestinal cancer organoids derived from cells or tissues, providing support for disease model construction for intestinal cancer, precision medicine research, and physiological function research of intestinal cancer. |
| Intended Use | For the efficient establishment and long-term culture of human intestinal cancer organoids derived from cells or tissues, supporting disease model construction, precision medicine, and physiological function research. For research use only. |
| Principle / Technology | Organoid culture (three-dimensional cell culture). The basement membrane matrix polymerizes at room temperature to form a biologically active 3D matrix that mimics the structure and function of the in vivo basement membrane, providing the three-dimensional environment required for organoid construction. The serum-free growth medium supplies proprietary components and cytokines that promote rapid intestinal cancer cell growth and stable organoid formation. |
| Sample Type | Cells and tissues of human origin |
| Performance Range / Specifications | Complete and convenient combination: the basement membrane matrix and growth medium components are supplied together as an integrated kit. Efficient and stable: the serum-free formulation, combined with the validated basement membrane matrix, promotes rapid and stable organoid formation. |
| Components / Formulation | Component 1: Organoid culture basement membrane matrix (phenol red-free, LDEV-free, EHS tumor-derived). Main constituents: laminin, type IV collagen, heparan sulfate proteoglycan (HSPG), entactin (nidogen), and growth factors such as TGF-β and EGF. Sterile preparation. Component 2: Human Intestinal Cancer Organoid Growth Medium Plus — serum-free formulation containing proprietary components and abundant cytokines that promote rapid intestinal cancer cell growth and organoid formation. |
| Storage Conditions | Basement membrane matrix: store frozen at -20 °C or below; valid for 2 years. Thaw at 2-8 °C until fully melted before use. Avoid repeated freeze-thaw cycles; it is recommended to aliquot by single-use volume after the first thaw and store the aliquots frozen at -20 °C. Growth medium: store frozen at -20 °C or below; valid for 1 year. Ready to use after thawing at 2-8 °C. It is recommended to use up within 3 months; aliquot any remaining medium by single-use volume and store frozen to avoid repeated freeze-thaw cycles. |
| Shelf Life | Basement membrane matrix: 2 years at -20 °C or below. Growth medium: 1 year at -20 °C or below (use within 3 months after thawing is recommended). |
| Package Specifications | 1 KIT |
| Product Form | Liquid kit components (basement membrane matrix and serum-free growth medium), supplied frozen or refrigerated as specified. |
| Quality Control | Sterile preparation. Basement membrane matrix: phenol red-free, LDEV-free. Growth medium: serum-free formulation. Validated for organoid culture. |
| Source / Origin | Basement membrane matrix: extracted from Engelbreth-Holm-Swarm (EHS) mouse tumors. Growth medium and organoids: human (Homo sapiens) applications. |
| Regulatory / Compliance | For research use only. |
| Application Notes / Precautions | Thaw the basement membrane matrix at 2-8 °C until fully melted before use. Avoid repeated freeze-thaw cycles; aliquot by single-use volume after the first thaw. It is recommended to use up the growth medium within 3 months after thawing; aliquot and freeze any remainder. For research use only. |
For research use only, not for clinical use.
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