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| Product Name | Human Esophageal Cancer Organoid Culture Kit |
| Catalog No. | RESK-0706 |
| Description | The Human Esophageal Cancer Organoid Culture Kit from Alta DiagnoTech is a complete, ready-to-use system for the efficient establishment and long-term culture of human esophageal cancer organoids derived from cells or tissues. The kit consists of two parts: an organoid culture basement membrane matrix (phenol red-free, LDEV-free, EHS tumor-derived) and a serum-free esophageal cancer organoid growth medium Plus containing proprietary components and a rich blend of cytokines. The matrix is a sterile preparation extracted from EHS mouse tumors. It mainly contains laminin, type IV collagen, heparan sulfate proteoglycan (HSPG), entactin, and growth factors such as TGF-beta and EGF. At room temperature, the matrix polymerizes to form a biologically active three-dimensional scaffold that mimics the structure and function of the in vivo basement membrane. The matrix has been validated for organoid culture and provides the three-dimensional support required for organoid construction. The growth medium is a serum-free formulation with proprietary components and abundant cytokines that enable esophageal cancer cells to grow rapidly and form organoids in an efficient and stable process while maintaining good cellular characteristics and viability. The kit is intended for the efficient establishment and long-term culture of esophageal cancer organoids from cells or tissues, supporting downstream physiological function research, disease model construction, and precision medicine. |
| Intended Use | For the efficient establishment and long-term culture of human esophageal cancer organoids derived from cells or tissues, supporting physiological function research, disease model construction, and precision medicine. For research use only. |
| Principle / Technology | Organoid culture technology based on a three-dimensional basement membrane matrix. The EHS tumor-derived matrix polymerizes at room temperature to form a biologically active 3D scaffold mimicking the in vivo basement membrane; combined with a serum-free, cytokine-rich growth medium, it supports rapid growth of cancer cells and stable organoid formation while preserving cellular characteristics and viability. |
| Sample Type | Human cells and tissues |
| Performance Range / Specifications | 1 KIT Complete, convenient combination of matrix and medium components supplied together Efficient and stable: serum-free formulation combined with a validated matrix promotes rapid and steady organoid formation |
| Specificity | Optimized for human esophageal cancer cells and tissues; species applicability: human. |
| Reaction Conditions / Protocol | Thaw the basement membrane matrix at 2-8 °C until completely liquefied before use. The matrix polymerizes at room temperature to form a three-dimensional gel; embed or overlay cancer cells/tissue fragments and culture with the serum-free organoid growth medium for establishment and long-term passaging of organoids. |
| Components / Formulation | Part 1: Organoid culture basement membrane matrix (phenol red-free, LDEV-free, EHS tumor-derived); sterile; mainly contains laminin, type IV collagen, heparan sulfate proteoglycan (HSPG), entactin, and growth factors such as TGF-beta and EGF Part 2: Serum-free esophageal cancer organoid growth medium Plus, containing proprietary components and abundant cytokines |
| Storage Conditions | Part I: store at 2-8 °C protected from light Part II: store at -25 to -15 °C Part III: store at -25 to -15 °C |
| Shelf Life | Part I and Part II: 12 months; Part III: 24 months (as stated on the product page) |
| Package Specifications | 1 KIT |
| Product Form | Liquid kit: basement membrane matrix and serum-free growth medium |
| Quality Control | Sterile preparation; phenol red-free; LDEV-free; matrix validated for organoid culture to support the three-dimensional environment required for organoid construction. |
| Source / Origin | Basement membrane matrix extracted from EHS (Engelbreth-Holm-Swarm) mouse tumors; growth medium is a chemically formulated serum-free preparation. |
| Expiration Date / Stability | Part I and Part II: 12 months; Part III: 24 months (as stated on the product page) |
| Regulatory / Compliance | For research use only. |
| Compatibility | Compatible with human cell- and tissue-derived samples for organoid establishment and long-term culture. |
| Application Notes / Precautions | Thaw the matrix at 2-8 °C until completely melted before use. Avoid repeated freeze-thaw cycles; aliquot into single-use portions after the first thaw and store frozen. Protect light-sensitive components from light during storage. |
For research use only, not for clinical use.
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