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| Product Name | Human Colorectal Organoid Growth Medium |
| Catalog No. | RESK-0659 |
| Description | Human Colorectal Organoid Growth Medium is a serum-free medium designed for the establishment and long-term culture of colorectal organoids derived from human cells or tissues. In the presence of an extracellular matrix, the proprietary components and abundant cytokines in the medium promote the rapid growth of colorectal cells and the formation of colorectal organoids. Organoid formation is smooth and rapid while maintaining high colorectal cell characteristics and viability, providing support for subsequent physiological function studies, disease research, and precision medicine based on colorectal organoids. Key features: 1. Broad application: suitable for organoid modeling, drug efficacy evaluation, toxicity assessment, and more. 2. Efficient and stable culture performance while preserving tissue heterogeneity. |
| Intended Use | Establishment and long-term culture of human colorectal organoids for organoid modeling, drug efficacy evaluation, toxicity assessment, disease research, and precision medicine. |
| Principle / Technology | Organoid culture technology. This serum-free complete medium contains proprietary components and a rich blend of cytokines that, in the presence of an extracellular matrix (basement membrane matrix), drive colorectal cells to grow rapidly and self-organize into colorectal organoids. An extracellular matrix is required for culture. |
| Sample Type | Human cells and tissues |
| Performance Range / Specifications | 1. Broad application: suitable for organoid modeling, drug efficacy evaluation, toxicity assessment, and more. 2. Efficient and stable culture performance while preserving tissue heterogeneity. |
| Reaction Conditions / Protocol | Culture colorectal organoids using an organoid-specific basement membrane matrix together with this medium: 1. Embed isolated colorectal cells or processed tissue fragments in the basement membrane matrix and allow the matrix to solidify. 2. Overlay the matrix with this growth medium and maintain the culture. 3. Refresh the medium and passage the organoids as required for long-term expansion. |
| Components / Formulation | Serum-free complete medium containing proprietary components and abundant cytokines. An extracellular matrix is required for organoid culture. |
| Storage Conditions | Store frozen at -20 °C or below. Avoid repeated freeze-thaw cycles. Thaw at 2-8 °C until completely melted before use. If the medium cannot be used up within a short period (3 months), aliquot it for storage to avoid repeated freeze-thaw cycles. |
| Shelf Life | 12 months |
| Package Specifications | 100 mL, 500 mL |
| Product Form | Liquid |
| Source / Origin | Human |
| Expiration Date / Stability | Stable for 12 months when stored frozen at -20 °C or below. After thawing at 2-8 °C, use promptly; if not used up within 3 months, aliquot and store to avoid repeated freeze-thaw cycles. |
| Regulatory / Compliance | For research use only. |
| Compatibility | For organoid culture, use together with an organoid-specific basement membrane matrix (extracellular matrix). |
| Application Notes / Precautions | 1. Avoid repeated freeze-thaw cycles; aliquot for storage if the medium will not be used up within 3 months after thawing. 2. Thaw completely at 2-8 °C before use. 3. For research use only. |
For research use only, not for clinical use.
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