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| Product Name | Human Colorectal Organoid Differentiation Medium |
| Catalog No. | RESK-0660 |
| Description | Human Colorectal Organoid Differentiation Medium is used for the differentiation culture of human colorectal organoids, yielding mature human colorectal organoids that contain all intestinal epithelial cell types. The establishment and maintenance culture of human colorectal organoids require the use of Human Colorectal Organoid Growth Medium. Key features: 1. Broad application: suitable for organoid modeling, drug efficacy evaluation, toxicity assessment, and more. 2. Efficient and stable culture performance while preserving tissue heterogeneity. |
| Intended Use | Differentiation culture of human colorectal organoids to obtain mature human colorectal organoids containing all intestinal epithelial cell types; suitable for organoid modeling, drug efficacy evaluation, and toxicity assessment. |
| Principle / Technology | Organoid differentiation culture technology. This medium directs expanded human colorectal organoids toward a mature state comprising the full spectrum of intestinal epithelial cell types, in contrast to the growth medium used for organoid establishment and maintenance. |
| Sample Type | Human organoids |
| Performance Range / Specifications | 1. Broad application: suitable for organoid modeling, drug efficacy evaluation, toxicity assessment, and more. 2. Efficient and stable culture performance while preserving tissue heterogeneity. |
| Reaction Conditions / Protocol | 1. Establish and maintain human colorectal organoids using Human Colorectal Organoid Growth Medium in an organoid-specific basement membrane matrix. 2. Switch the expanded organoids to this differentiation medium and continue culture in the basement membrane matrix. 3. Maintain the differentiation culture until mature human colorectal organoids containing all intestinal epithelial cell types are obtained. |
| Components / Formulation | Liquid medium supplied as two components: Part I and Part II. Part I: store at 2-8 °C, protected from light. Part II: store at -25 °C to -15 °C. |
| Storage Conditions | Part I: Store at 2-8 °C, protected from light. Part II: Store at -25 °C to -15 °C. |
| Shelf Life | 12 months |
| Package Specifications | 100 mL, 500 mL |
| Product Form | Liquid |
| Source / Origin | Human |
| Shipping Conditions | Part I: Shipped with ice packs. Part II: Shipped with dry ice. |
| Regulatory / Compliance | For research use only. |
| Compatibility | Establishment and maintenance culture of human colorectal organoids require Human Colorectal Organoid Growth Medium. For organoid culture, use together with an organoid-specific basement membrane matrix (extracellular matrix). |
| Application Notes / Precautions | 1. Store Part I protected from light at 2-8 °C and Part II at -25 °C to -15 °C. 2. Use Human Colorectal Organoid Growth Medium for organoid establishment and expansion before switching to this differentiation medium. 3. For research use only. |
For research use only, not for clinical use.
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