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| Product Name | HT Media Supplement, 50×, Sterile |
| Catalog No. | RERE-0401 |
| Description | Hypoxanthine and thymidine are the rescue nutrients of salvage-pathway biology: cells cut off from de novo nucleotide synthesis can still divide if the medium feeds their salvage enzymes directly. This sterile 50× blend packs both substrates into a concentrate from which 10 mL prepares 500 mL of working medium. Laboratories deploy it during HAT selection stages that need the selective block lifted, and for general rescue of cultures under nucleotide stress. |
| Intended Use | Supplying salvage-pathway substrates during hybridoma selection workflows and recovery of stressed cultures. |
| Principle / Technology | Exogenous hypoxanthine and thymidine bypass aminopterin-imposed blocks by feeding nucleotide salvage routes. |
| Specificity | 50× concentrate; hypoxanthine and thymidine; sterile solution; 10 mL prepares 500 mL of medium. |
| Reaction Conditions / Protocol | Dilute 1:50 into complete medium aseptically according to the selection or rescue scheme. |
| Storage Conditions | -20°C; protected from light; 12 months. |
| Package Specifications | 10 mL, 10 × 10 mL |
| Key Features | Two-component salvage nutrient blend; sterile 50× stock; flexible for HAT and standalone HT protocols. |
For research use only, not for clinical use.
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