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| Product Name | HpaII Rapid Restriction Endonuclease (20 U/uL) |
| Catalog No. | IVDRM-0426 |
| Description | Recognising the CpG containing site 5 prime C^CGG 3 prime, this rapid recombinant endonuclease is blocked by CpG methylation, making it a standard partner in methylation sensitive restriction analysis alongside its MspI counterpart. It supports plasmid linearisation and vector construction with digestion of plasmid DNA, PCR products and genomic DNA in 5 to 15 minutes. Supplied at 20 U/uL, it runs in the common rapid series buffer at 37°C with heat inactivation at 80°C for 20 minutes, and the isoschizomers MspI, HapII and BsiSI share the specificity. |
| Intended Use | Plasmid linearisation, vector construction and CpG methylation sensitive restriction analysis |
| Principle / Technology | Site specific cleavage at 5 prime C^CGG 3 prime within 5 to 15 minutes at 37°C |
| Specificity | CpG methylation blocks cleavage; isoschizomers MspI, HapII and BsiSI |
| Reaction Conditions / Protocol | Digest in 1x rapid restriction buffer at 37°C for 5 to 15 minutes; heat inactivate at 80°C for 20 minutes |
| Storage Conditions | -25 to -15°C |
| Shelf Life | 2 years |
| Package Specifications | 200 T |
| Key Features | Methylation sensitive counterpart to MspI for epigenetic workflows; unified buffer across the series |
| Concentration | 20 U/uL |
| Readouts / Downstream Assays | Methylation sensitive restriction pattern on agarose gel |
For in vitro diagnostic use.
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