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| Product Name | Hotstart Taq DNA Polymerase, Ultra-Low Residual (20 U/uL) |
| Catalog No. | IVDRM-0505 |
| Description | A hot-start Taq DNA polymerase blocked by two proprietary monoclonal antibodies that close down both the polymerase activity and the exonuclease activity until a 30 second pre-denaturation heat step releases them. The double blocking design suppresses non-specific amplification from mismatches or primer dimers and protects against fluorescence signal loss caused by probe degradation, keeping assays reliable during transport and room temperature handling. Processed under an ultra-low residual production regime, the enzyme carries minimal nuclease and host genomic DNA residue, below 0.02 copies per 100 U, and a fully pre-mixed reaction stays stable for 7 days at 37°C, which suits high sensitivity, low residual in vitro diagnostic system development. |
| Intended Use | PCR and real-time quantitative PCR enzyme raw material for IVD molecular diagnostic and in vitro detection assay development |
| Principle / Technology | Dual antibody blocked hot-start Taq polymerase released at pre-denaturation with both catalytic activities restored on heating |
| Sample Type | DNA templates for PCR and real-time quantitative PCR |
| Reaction Conditions / Protocol | Activate with 30 seconds of pre-denaturation heating, then run the PCR or qPCR cycling programme |
| Storage Conditions | -25 to -15°C |
| Shelf Life | 2 years |
| Package Specifications | 1000 U; 10000 U; 25000 U; 100000 U |
| Quality Control | Hot start by dual antibody blocking; ultra-low residual process; fully pre-mixed mix stable for 7 days at 37°C |
| Key Features | Double blocking design; stable fully pre-mixed format; ultra-low nuclease and host genomic DNA residue |
| Purity | Host genomic DNA below 0.02 copies per 100 U |
| Concentration | 20 U/uL |
| Readouts / Downstream Assays | Real-time quantitative PCR amplification curves |
For in vitro diagnostic use.
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