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| Product Name | Hotstart Taq DNA Polymerase for Multiplex and High-Sensitivity PCR (5 U/uL) |
| Catalog No. | IVDRM-0506 |
| Description | An antibody-blocked thermostable mutant Taq DNA Polymerase whose activity stays suppressed even at 60°C and is released only after heating to 95°C, preventing the non-specific amplification and primer dimer formation that otherwise arise during sample preparation and reaction heating. Like wild type Taq DNA polymerase it carries 5 prime to 3 prime polymerase and 5 prime to 3 prime exonuclease activities but lacks 3 prime to 5 prime exonuclease activity. These properties suit high-sensitivity nucleic acid amplification and simultaneous amplification of multiple targets in one reaction. |
| Intended Use | High-sensitivity PCR and multiplex amplification of several targets in one reaction |
| Principle / Technology | Antibody blocked hot-start mutant Taq polymerase released on heating to 95°C |
| Sample Type | Genomic DNA, cDNA and plasmid DNA templates for multiplex amplification |
| Reaction Conditions / Protocol | Assemble reactions on ice, then activate by heating to 95°C before cycling |
| Storage Conditions | -25 to -15°C |
| Shelf Life | 2 years |
| Package Specifications | 250 U; 500 U; 1000 U; 10000 U; 25000 U |
| Quality Control | Antibody blocked hot start with activity still suppressed at 60°C |
| Key Features | Hot start active even at 60°C; 5 prime to 3 prime polymerase and exonuclease activities without proofreading |
| Purity | Free of exonuclease, endonuclease and RNase residue |
| Concentration | 5 U/uL |
| Readouts / Downstream Assays | Agarose gel electrophoresis of multiplex amplicons |
For in vitro diagnostic use.
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