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| Product Name | HotStart Taq DNA Polymerase, Antibody-Modified Wild Type (5 U/uL) |
| Catalog No. | IVDRM-0564 |
| Description | A mixture of a high affinity Taq monoclonal antibody and a thermostable recombinant wild-type Taq DNA polymerase with strong template affinity. The antibody keeps the polymerase blocked even after 30 minutes at 50°C, and heating for 30 seconds at the pre-denaturation temperature fully releases the DNA polymerase activity, so amplification caused by non-specific primer annealing during reaction setup and heating is effectively suppressed. Like the parent enzyme it carries 5 prime to 3 prime polymerase and exonuclease activities without proofreading, and the 3 prime adenosine overhangs of its products allow direct cloning into T-vectors. The linear detection range spans ten to the seventh down to ten to the third copies, supporting hot-start PCR, qPCR, multiplex amplification and low copy template detection. |
| Intended Use | Hot-start PCR, qPCR, multiplex amplification, low copy template detection and T-vector cloning |
| Principle / Technology | Antibody-sequestered wild-type Taq polymerase released at pre-denaturation for hot-start amplification |
| Sample Type | Genomic DNA templates and PCR products for T-vector cloning |
| Specificity | Antibody remains blocking after 30 minutes at 50°C; products carry 3 prime dA overhangs; linear range 10 to the 3 to 10 to the 7 copies |
| Reaction Conditions / Protocol | Assemble on ice and activate with 30 seconds of pre-denaturation heating before cycling |
| Storage Conditions | -25 to -15°C |
| Shelf Life | 2 years |
| Package Specifications | 250 U; 500 U; 1000 U; 10000 U; 25000 U; 100,000 U |
| Key Features | Wild type enzyme performance with antibody hot start; T-vector cloning ready products |
| Purity | No residual exonuclease activity |
| Concentration | 5 U/uL |
| Readouts / Downstream Assays | Agarose gel electrophoresis of amplified products |
For in vitro diagnostic use.
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