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| Product Name | Hotstart E-Taq DNA Polymerase, Ultra-Low Residual (20 U/uL) |
| Catalog No. | IVDRM-0504 |
| Description | A hot-start DNA polymerase blocked by two proprietary monoclonal antibodies that shut down both the 5 prime to 3 prime polymerase activity and the 5 prime to 3 prime exonuclease activity. Heating for 30 seconds at pre-denaturation temperature fully releases both activities. The double blocking design prevents non-specific amplification caused by mismatches or primer dimers and suppresses the fluorescence signal drop that probe degradation would otherwise cause, keeping in vitro detection assays stable during transport and room temperature use. An ultra-low residual production process keeps nuclease and host genomic DNA residue extremely low, below 0.02 copies per 100 U, and with an optimised buffer system the enzyme supports qPCR systems in which primers and probes are pre-mixed in advance. |
| Intended Use | Development of probe-based qPCR and IVD molecular diagnostic systems with primers and probes pre-mixed in advance |
| Principle / Technology | Dual antibody blocked hot-start Taq polymerase released at pre-denaturation, with both polymerase and exonuclease activities restored on heating |
| Sample Type | DNA templates for real-time quantitative PCR |
| Reaction Conditions / Protocol | Activate with 30 seconds of pre-denaturation heating, then run the qPCR cycling programme with the optimised buffer system |
| Storage Conditions | -25 to -15°C |
| Shelf Life | 2 years |
| Package Specifications | 1000 U; 10000 U; 25000 U; 100000 U |
| Quality Control | Hot start by dual antibody blocking; ultra-low residual process with minimal nuclease and host genomic DNA residue |
| Key Features | Double blocking prevents non-specific amplification and probe degradation signal loss; supports fully pre-mixed qPCR systems |
| Purity | Host genomic DNA below 0.02 copies per 100 U |
| Concentration | 20 U/uL |
| Readouts / Downstream Assays | Real-time quantitative PCR amplification curves |
For in vitro diagnostic use.
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