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| Product Name | Hotstart E-Taq DNA Polymerase (5 U/uL) |
| Catalog No. | IVDRM-0563 |
| Description | A dual-blocked hot-start Taq DNA polymerase developed through rational design and directed evolution of DNA polymerases, with advantageous mutants obtained by site mutagenesis, random mutagenesis screening, stress testing and enzyme fragment recombination. A proprietary antibody blocks both the 5 prime to 3 prime polymerase activity and the 5 prime to 3 prime exonuclease activity, and heating for 30 seconds at the pre-denaturation temperature fully releases both activities. The double blocking design prevents non-specific amplification from mismatches or primer dimers and suppresses the fluorescence signal drop caused by probe degradation. Amplification efficiency reaches 99% from five million down to 50 copies per reaction with R2 of 0.99, the detection limit is 5 copies per reaction, and a fully pre-mixed qPCR system tolerates 14 days at 37°C and 50 freeze-thaw cycles. |
| Intended Use | Low copy gene detection from complex genomic or cDNA templates and multiplex qPCR such as four-plex respiratory targets |
| Principle / Technology | Dual antibody blocked hot-start Taq polymerase with both polymerase and exonuclease activities restored on heating |
| Sample Type | Genomic DNA and cDNA templates including low copy targets |
| Specificity | Amplification efficiency 99% with R2 of 0.99; detection limit 5 copies per reaction |
| Reaction Conditions / Protocol | Activate with 30 seconds of pre-denaturation heating, then run the qPCR cycling programme |
| Storage Conditions | -25 to -15°C |
| Shelf Life | 2 years |
| Package Specifications | 250 U; 500 U; 1000 U; 10000 U; 25000 U; 100000 U |
| Key Features | Fully pre-mixed system stable for 14 days at 37°C and 50 freeze-thaw cycles |
| Purity | Free of exonuclease, endonuclease and RNase residue |
| Concentration | 5 U/uL |
| Readouts / Downstream Assays | Real-time qPCR amplification curves across the dilution series |
For in vitro diagnostic use.
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