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| Product Name | HotStart Direct Taq DNA Polymerase for Blood and Swab Samples (5 U/uL) |
| Catalog No. | IVDRM-0562 |
| Description | A hot-start DNA polymerase that tolerates blood and other PCR inhibitors, Direct Taq uses antibody blocking with good amplification sensitivity and specificity. Heating for 30 seconds at the pre-denaturation temperature fully inactivates the antibody and releases the DNA polymerase activity, and the hot-start behaviour suppresses amplification caused by non-specific primer annealing. The enzyme tolerates EDTA, sodium citrate and 0.5% whole blood in the reaction, and a qPCR mix prepared with it stays stable for 7 days at both 4°C and 37°C, which supports direct amplification from blood and swab samples in IVD molecular diagnostic PCR and qPCR assays. |
| Intended Use | High sensitivity and specific PCR and qPCR directly from blood and swab samples in IVD molecular diagnostics |
| Principle / Technology | Antibody blocked hot-start Taq polymerase released after 30 seconds of pre-denaturation heating |
| Sample Type | Whole blood, swab eluates and other inhibitor-containing templates |
| Specificity | Tolerates EDTA, sodium citrate and 0.5% whole blood; qPCR mix stable for 7 days at 4 and 37°C |
| Reaction Conditions / Protocol | Assemble the reaction directly with the sample and activate with 30 seconds of pre-denaturation heating |
| Storage Conditions | -20°C |
| Shelf Life | 2 years |
| Package Specifications | 250 U; 500 U; 1000 U; 10000 U; 25000 U; 50000 U |
| Key Features | Direct amplification from inhibitor-rich samples; stable prepared mixes for room temperature handling |
| Purity | Free of exonuclease, endonuclease and RNase residue |
| Concentration | 5 U/uL |
| Readouts / Downstream Assays | Real-time PCR amplification curves from direct samples |
For in vitro diagnostic use.
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