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| Product Name | HinP1I Rapid Restriction Endonuclease (10 U/uL) |
| Catalog No. | IVDRM-0429 |
| Description | This rapid recombinant endonuclease cleaves 5 prime G^CGC 3 prime and supports recombinant vector construction and molecular cloning, digesting plasmid DNA, PCR products and genomic DNA within 5 to 15 minutes. Supplied at 10 U/uL, it operates in the single buffer common to the rapid series at 37°C and is heat inactivated at 80°C for 20 minutes. CpG methylation at the site influences cutting, and the enzyme shares its specificity with the isoschizomers HhaI, CfoI, AspLEI, Hin6I, HspAI and BstHHI. |
| Intended Use | Molecular cloning and recombinant vector construction with rapid digestion |
| Principle / Technology | Site specific cleavage at 5 prime G^CGC 3 prime within 5 to 15 minutes at 37°C |
| Specificity | CpG methylation affects cleavage; isoschizomers include HhaI, CfoI, AspLEI, Hin6I, HspAI and BstHHI |
| Reaction Conditions / Protocol | Digest in 1x rapid restriction buffer at 37°C for 5 to 15 minutes; heat inactivate at 80°C for 20 minutes |
| Storage Conditions | -25 to -15°C |
| Shelf Life | 2 years |
| Package Specifications | 100 T |
| Key Features | One shared buffer across the rapid series; tolerant of excess substrate and difficult templates |
| Concentration | 10 U/uL |
| Readouts / Downstream Assays | Gel profile of digested DNA |
For in vitro diagnostic use.
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