HinfI Rapid Restriction Endonuclease (50 U/uL)
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HinfI Rapid Restriction Endonuclease (50 U/uL)

Cat.No: IVDRM-0425 Datasheet

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Product Details Related Products
Product Name HinfI Rapid Restriction Endonuclease (50 U/uL)
Catalog No. IVDRM-0425
Description Targeting the degenerate sequence 5 prime G^ANTC 3 prime, this rapid recombinant endonuclease cuts plasmid DNA, PCR products and genomic DNA within 5 to 15 minutes and suits fragment preparation for cloning. Supplied at a high 50 U/uL, it operates in the shared rapid series buffer at 37°C and is heat inactivated at 80°C for 20 minutes. CpG and EcoBI methylation possibly overlapping the site block cleavage, and no alternative named isoschizomers are documented.
Intended Use Rapid cutting of plasmid DNA, PCR products and genomic DNA for fragment preparation
Principle / Technology Site specific cleavage at 5 prime G^ANTC 3 prime within 5 to 15 minutes at 37°C
Specificity CpG and EcoBI methylation may block cleavage; no alternative named isoschizomers
Reaction Conditions / Protocol Digest in 1x rapid restriction buffer at 37°C for 5 to 15 minutes; heat inactivate at 80°C for 20 minutes
Storage Conditions -25 to -15°C
Shelf Life 2 years
Package Specifications 500 T
Key Features Large 500 reaction pack; one shared buffer for the whole rapid series
Concentration 50 U/uL
Readouts / Downstream Assays Restriction digest pattern on agarose gel

For in vitro diagnostic use.

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