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| Product Name | HindIII Rapid Restriction Endonuclease, High Concentration (100 U/uL) |
| Catalog No. | IVDRM-0423 |
| Description | Recognising the classic site 5 prime A^AGCTT 3 prime, this high concentration rapid recombinant endonuclease digests plasmid DNA, PCR products and genomic DNA within 5 to 15 minutes. Supplied at 100 U/uL, the concentrated format reduces glycerol carryover and suits automation friendly workflows, and it runs in the single buffer shared across the rapid series. Heat inactivation takes 20 minutes at 80°C EcoBI methylation possibly overlapping the site may block cutting, and no alternative named isoschizomers are documented. This unit stores at -20°C and ships with ice packs. |
| Intended Use | Rapid DNA cutting for molecular cloning with concentrated enzyme for automation friendly setups |
| Principle / Technology | Site specific cleavage at 5 prime A^AGCTT 3 prime within 5 to 15 minutes at 37°C |
| Specificity | EcoBI methylation may block cleavage; no alternative named isoschizomers |
| Reaction Conditions / Protocol | Digest in 1x rapid restriction buffer at 37°C for 5 to 15 minutes; heat inactivate at 80°C for 20 minutes |
| Storage Conditions | -20°C |
| Shelf Life | 2 years |
| Package Specifications | 500 T |
| Key Features | High concentration 100 U/uL format; single shared buffer across the rapid series |
| Concentration | 100 U/uL |
| Shipping Conditions | Ice packs |
| Readouts / Downstream Assays | Agarose gel profile of digested DNA |
For in vitro diagnostic use.
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