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| Product Name | High-Concentration Tn5 Transposase for ATAC and Tagmentation Library Prep |
| Catalog No. | IVDRM-0331 |
| Description | A high-concentration, mutant Tn5 transposase supplied as a bare enzyme at 10 U/uL protein concentration 2.2 ug/uL. Recognising the inside, outside and mosaic end sequences of the Tn5 transposon, with the mosaic end giving the highest transposition efficiency, the enzyme inserts adapter-loaded transposons into target DNA rapidly and near-randomly once adapters are assembled onto it. Applications span sequencing library construction, ATAC chromatin accessibility profiling, bacterial mutant library construction, methylation studies, single-cell genome analysis and long-read genome projects. |
| Intended Use | Tagmentation-based sequencing library construction; ATAC chromatin accessibility profiling; bacterial mutant library construction; methylation and single-cell genomic workflows; long-read genome projects. |
| Principle / Technology | Adapter-loaded mutant Tn5 transposase fragments and tags DNA in one step by inserting transposon ends across the target. |
| Sample Type | Genomic DNA, chromatin, single-cell material |
| Performance Range / Specifications | 10 U/uL enzyme at 2.2 ug/uL; high-efficiency random insertion; ME-containing fragments transpose most efficiently. |
| Specificity | Bare enzyme format for custom adapter assembly. |
| Reaction Conditions / Protocol | Load adapters onto the enzyme, combine with target DNA under tagmentation conditions and amplify libraries. |
| Components / Formulation | High-concentration Tn5 transposase with storage buffer. |
| Storage Conditions | Enzyme component at -85 to -65 °C; assembly products and remaining components at -25 to -15 °C |
| Package Specifications | 10 uL, 50 uL |
| Product Form | Bare enzyme solution |
| Key Features | High concentration for demanding tagmentation; custom adapter flexibility; broad application range. |
| Concentration | 10 U/uL; 2.2 ug/uL |
| Source / Origin | Recombinant mutant Tn5 transposase |
| Readouts / Downstream Assays | Tagmented libraries for accessibility and genomic analysis |
For in vitro diagnostic use.
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