- Home
- IVD
- By Technology Types
- By Diseases Types
- By Product Types
- Research
- Resource
- Distributors
- Company
| Product Name | HiFi Cas9 Nuclease, High-Fidelity SpCas9 Variant (5 mg/mL) |
| Catalog No. | IVDRM-0584 |
| Description | An engineered variant of Streptococcus pyogenes SpCas9, HiFi Cas9 carries a series of point mutations that weaken the interaction between Cas9 and the non-target DNA strand, raising specificity so that off-target effects often fall below the limit of detection while on-target activity is preserved. The protein carries two nuclear localisation signals and a C-terminal 6-His tag and is intended for ribonucleoprotein delivery by electroporation, microinjection or lipofection. It can replace standard Cas9 Nuclease directly and is recommended for off-target sensitive work such as stem cell editing, pre-therapeutic studies and in vivo experiments. Purity is at least 95%. |
| Intended Use | Off-target sensitive genome editing including stem cell editing, pre-therapeutic studies and in vivo work |
| Principle / Technology | Engineered SpCas9 variant with a weakened non-target strand interaction that raises specificity |
| Sample Type | Cells and embryos edited through Cas9 ribonucleoprotein delivery |
| Specificity | High fidelity with off-target effects often below the limit of detection and preserved on-target activity |
| Reaction Conditions / Protocol | Deliver as a ribonucleoprotein complex by electroporation, microinjection or lipofection |
| Storage Conditions | -25 to -15°C |
| Shelf Life | 2 years |
| Package Specifications | 100 ug; 1 mg |
| Key Features | Dual nuclear localisation signals with a C-terminal 6-His tag; direct replacement for standard Cas9 Nuclease |
| Purity | Greater than or equal to 95% |
| Concentration | 5 to 6 mg/mL |
| Source / Origin | Streptococcus pyogenes |
| Readouts / Downstream Assays | In vitro cleavage assay and editing efficiency at the target locus |
For in vitro diagnostic use.
|
There is no product in your cart. |
Copyright © 2026 Alta DiagnoTech. All rights reserved.
