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| Product Name | HhaI Rapid Restriction Endonuclease (20 U/uL) |
| Catalog No. | IVDRM-0428 |
| Description | Recognising 5 prime GCG^C 3 prime, this rapid recombinant endonuclease serves vector construction and molecular cloning with digestion of plasmid DNA, PCR products and genomic DNA in 5 to 15 minutes. CpG methylation at the site influences cutting, a property exploited in methylation sensitive restriction analysis. The enzyme is supplied at 20 U/uL, digests in the shared rapid series buffer at 37°C, and is inactivated by heating at 80°C for 20 minutes. Its isoschizomer panel includes HinP1I, CfoI, AspLEI, Hin6I, HspAI and BstHHI, while Dam, Dcm, EcoKI and EcoBI methylation are tolerated. |
| Intended Use | Vector construction, molecular cloning and methylation sensitive restriction analysis at GCGC sites |
| Principle / Technology | Site specific cleavage at 5 prime GCG^C 3 prime within 5 to 15 minutes at 37°C |
| Specificity | CpG methylation affects cleavage; Dam, Dcm, EcoKI and EcoBI have no effect; isoschizomers include HinP1I, CfoI, AspLEI, Hin6I, HspAI and BstHHI |
| Reaction Conditions / Protocol | Digest in 1x rapid restriction buffer at 37°C for 5 to 15 minutes; heat inactivate at 80°C for 20 minutes |
| Storage Conditions | -25 to -15°C |
| Shelf Life | 2 years |
| Package Specifications | 100 T |
| Key Features | Methylation sensitive cutter with broad isoschizomer compatibility; unified buffer across the series |
| Concentration | 20 U/uL |
| Readouts / Downstream Assays | Digestion fragment pattern on agarose gel |
For in vitro diagnostic use.
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