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| Product Name | HEPES Buffered Saline, 1 M, pH 7.4, Sterile |
| Catalog No. | ATR-CCR-0017 |
| Description | A 1 M sterile HEPES buffer solution for additional pH buffering of cell culture media during extended incubations outside the CO2 incubator, and for adjusting medium formulations. |
| Intended Use | Supplementing cell culture media with additional buffering capacity for experiments conducted outside CO2 incubators; pH adjustment in custom medium formulations. |
| Principle / Technology | HEPES zwitterionic buffer provides pH stability independent of CO2 levels, unlike the bicarbonate system; pKa of 7.55 at 25°C makes it ideal for physiological pH maintenance during extended cell manipulations at ambient conditions. |
| Detection Method | pH electrode, cell viability in ambient air conditions |
| Sample Type | Cell culture media requiring additional pH control |
| Performance Range / Specifications | 1 M stock; typical working concentration 10–25 mM; provides pH 7.2–7.6 at 37°C |
| Sensitivity / LOD | N/A |
| Specificity | N/A |
| Reaction Conditions / Protocol | Add 5–25 mL per liter of medium to achieve 5–25 mM final concentration; adjust medium pH if necessary after supplementation. |
| Components / Formulation | HEPES free acid dissolved in cell culture grade water, pH adjusted to 7.4 with NaOH, sterile-filtered |
| Storage Conditions | 15–30°C |
| Shelf Life | 24 months |
| Package Specifications | 100 mL, 500 mL |
| Product Form | Liquid concentrate (1 M), sterile |
| Quality Control | pH, sterility, endotoxin, cell toxicity testing |
| Key Features | Sterile; CO2 independent buffering; cell culture tested; ready to use; extends pH stability during extended procedures |
For research use only, not for clinical use.
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