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| Product Name | HEK293T Human SLC7A11 & SLC3A2 Stable Cell Line |
| Catalog No. | ATR-CCR-0070 |
| Description | A cryopreserved, adherent HEK293T cell line stably expressing c-terminal flag-tagged and c-terminal 6xhis-tagged human SLC7A11 & SLC3A2. The cells are maintained under puromycin and blasticidin selection and are intended for cell-based research assays. |
| Intended Use | For FACS binding assays and cell-based ELISA. |
| Principle / Technology | Stable transfection followed by antibiotic selection; SLC7A11 & SLC3A2 expression is qualified using flow cytometry; cell-based ELISA. |
| Detection Method | Flow cytometry; cell-based ELISA |
| Sample Type | Stable mammalian cell line |
| Performance Range / Specifications | Approximately 5 × 10^6 cells in 1 mL per vial; adherent growth. |
| Specificity | Human SLC7A11 and SLC3A2 |
| Reaction Conditions / Protocol | Culture in DMEM with 10% FBS, 2 μg/mL puromycin and 2 μg/mL blasticidin. |
| Components / Formulation | Cryopreservation medium: 35% FBS, 55% DMEM and 10% DMSO. |
| Storage Conditions | Store frozen in liquid nitrogen or at -80°C. |
| Package Specifications | 1 vial; approximately 5 × 10^6 cells in 1 mL |
| Product Form | Cryopreserved stable cell line |
| Quality Control | SLC7A11 expression and tagged SLC3A2 expression verified by flow cytometry against parental HEK293T cells. |
| Key Features | Stable human SLC7A11 & SLC3A2 expression; adherent growth; puromycin and blasticidin resistance; c-terminal flag-tagged and c-terminal 6xhis-tagged; suitable for FACS binding assays and cell-based ELISA. |
| Source / Origin | HEK293T cells expressing human SLC7A11 & SLC3A2 (UniProt Q9UPY5 and P08195). |
| Regulatory / Compliance | For research use only; not for clinical diagnosis or treatment. |
| Compatibility | FACS binding assays and cell-based ELISA |
| Recommended Buffer System | DMEM with 10% FBS, 2 μg/mL puromycin and 2 μg/mL blasticidin |
| Application Notes / Precautions | Maintain puromycin and blasticidin selection during routine culture. Keep the product frozen until cell recovery. |
| Genotype | Stable human SLC7A11 and SLC3A2 co-expression; puromycin- and blasticidin-resistant. |
| Alternative Names / Synonyms | SLC7A11 & SLC3A2; Cystine/glutamate transporter subunit SLC7A11 and 4F2 cell-surface antigen heavy chain SLC3A2 |
| Host Species | Human |
| Tag Information | C-terminal Flag-tagged and C-terminal 6xHis-tagged |
For research use only, not for clinical use.
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