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| Product Name | HEK293T Human SARM1 Stable Cell Line |
| Catalog No. | ATR-CCR-0061 |
| Description | A cryopreserved, adherent HEK293T cell line stably expressing c-terminal flag-tagged human SARM1. The cells are maintained under puromycin selection and are intended for cell-based research assays. |
| Intended Use | For FACS binding assays and Western blotting. |
| Principle / Technology | Stable transfection followed by antibiotic selection; SARM1 expression is qualified using flow cytometry; Western blot. |
| Detection Method | Flow cytometry; Western blot |
| Sample Type | Stable mammalian cell line |
| Performance Range / Specifications | Approximately 5 × 10^6 cells in 1 mL per vial; adherent growth; observed Western blot band approximately 77 kDa. |
| Specificity | Human SARM1 |
| Reaction Conditions / Protocol | Culture in DMEM with 10% FBS and 2 μg/mL puromycin. |
| Components / Formulation | Cryopreservation medium: 35% FBS, 55% DMEM and 10% DMSO. |
| Storage Conditions | Store frozen in liquid nitrogen or at -80°C. |
| Package Specifications | 1 vial; approximately 5 × 10^6 cells in 1 mL |
| Product Form | Cryopreserved stable cell line |
| Quality Control | SARM1 expression verified by flow cytometry and Western blot. The reported band is approximately 77 kDa, consistent with the predicted 77.5 kDa size. |
| Key Features | Stable human SARM1 expression; adherent growth; puromycin resistance; c-terminal flag-tagged; suitable for FACS binding assays and Western blotting. Protein expression is supported by both flow-cytometric and immunoblot qualification. |
| Source / Origin | HEK293T cells expressing human SARM1 (UniProt Q6SZW1). |
| Regulatory / Compliance | For research use only; not for clinical diagnosis or treatment. |
| Compatibility | FACS binding assays and Western blotting |
| Recommended Buffer System | DMEM with 10% FBS and 2 μg/mL puromycin |
| Application Notes / Precautions | Maintain puromycin selection during routine culture. Keep the product frozen until cell recovery. |
| Genotype | Stable human SARM1 expression; puromycin-resistant. |
| Alternative Names / Synonyms | SARM1; NAD(+) hydrolase SARM1 |
| Host Species | Human |
| Tag Information | C-terminal Flag-tagged |
For research use only, not for clinical use.
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