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| Product Name | HEK293T Human CLDN6 Stable Cell Line |
| Catalog No. | ATR-CCR-0053 |
| Description | A puromycin-resistant HEK293T cell line stably expressing tag-free human CLDN6. The adherent cells are supplied cryopreserved and have been evaluated for CLDN6 expression by flow cytometry. |
| Intended Use | For binding assays by flow cytometry and cell-based ELISA. |
| Principle / Technology | Stable transfection followed by antibiotic selection; CLDN6 expression is verified by flow cytometry against parental HEK293T cells. |
| Detection Method | Flow cytometry; cell-based ELISA |
| Sample Type | Stable mammalian cell line |
| Performance Range / Specifications | Approximately 5 × 10^6 cells in 1 mL per vial; adherent growth. |
| Specificity | Human CLDN6 (UniProt P56747) |
| Reaction Conditions / Protocol | Culture in DMEM supplemented with 10% FBS and 0.75 µg/mL puromycin. |
| Components / Formulation | Cryopreservation medium: 35% FBS, 55% DMEM and 10% DMSO. |
| Storage Conditions | Store frozen in liquid nitrogen or at -80°C. |
| Package Specifications | 1 vial; approximately 5 × 10^6 cells in 1 mL |
| Product Form | Cryopreserved stable cell line |
| Quality Control | CLDN6 expression verified by flow cytometry. |
| Key Features | Stable human CLDN6 expression; adherent growth; puromycin resistance; tag-free protein; suitable for cell-based binding assays. |
| Source / Origin | HEK293T cells expressing human CLDN6 (UniProt P56747) |
| Regulatory / Compliance | For research use only; not for clinical diagnosis or treatment. |
| Compatibility | FACS binding assays; cell-based ELISA |
| Recommended Buffer System | DMEM with 10% FBS and 0.75 µg/mL puromycin |
| Application Notes / Precautions | Maintain puromycin selection during routine culture. Keep the product frozen until cell recovery. |
| Genotype | Stable human CLDN6 expression; puromycin-resistant |
| Alternative Names / Synonyms | CLDN6; Claudin-6 |
| Host Species | Human |
| Tag Information | Tag-Free |
For research use only, not for clinical use.
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