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| Product Name | HAT Media Supplement, 50×, Sterile |
| Catalog No. | RERE-0402 |
| Description | The complete HAT cocktail unites all three arms of the classic selection chemistry in one sterile 50× vial: hypoxanthine and thymidine feed the salvage pathway while aminopterin shuts down the de novo route, leaving only appropriately competent hybrids free to proliferate. Ten millilitres of this concentrate fortifies half a litre of complete medium, so a fusion experiment can be set up from a single bottle. |
| Intended Use | One-step establishment of HAT selection conditions for hybridoma generation and related cell lines. |
| Principle / Technology | Aminopterin blocks de novo nucleotide synthesis while hypoxanthine and thymidine support the salvage route, restricting growth to competent cells. |
| Specificity | 50× concentrate; hypoxanthine, aminopterin and thymidine; sterile solution; 10 mL prepares 500 mL of medium. |
| Reaction Conditions / Protocol | Dilute 1:50 into complete medium under aseptic conditions to impose selection. |
| Storage Conditions | -20°C; protected from light; 12 months. |
| Package Specifications | 10 mL, 10 × 10 mL |
| Key Features | All-in-one selection cocktail; sterile and protected from light; 500 mL of medium per vial. |
For research use only, not for clinical use.
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