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| Product Name | Ham's F12K Medium (Kaighn's Modification), with L-Glutamine |
| Catalog No. | ATR-CCR-0034 |
| Description | Ham's F12K medium, a modified version of Ham's F12 with increased amino acid concentrations and additional supplements specifically developed for the culture of primary human and other mammalian cells. The enriched formulation contains higher levels of several amino acids, putrescine, and additional vitamins compared to standard F12. It is particularly suitable for supporting the growth of primary human endothelial cells, keratinocytes, and various epithelial cell types. |
| Intended Use | Culture of primary human epithelial and endothelial cells with enriched amino acid and micronutrient requirements. |
| Principle / Technology | Kaighn's modified Ham's F12 nutrient mixture with putrescine and elevated amino acid levels |
| Detection Method | Phase contrast microscopy for morphology assessment; growth curve analysis |
| Sample Type | Primary human umbilical vein endothelial cells, epidermal keratinocytes, chondrocytes, and rat liver epithelial cells |
| Performance Range / Specifications | Contains all 20 amino acids at elevated concentrations; putrescine: 0.5 mg/L; L-glutamine: 2.5 mM |
| Sensitivity / LOD | Supports primary endothelial cell growth for 4–8 population doublings before senescence |
| Specificity | Formulated specifically for primary cell types with high amino acid requirements not met by standard MEM or DMEM |
| Reaction Conditions / Protocol | Supplement with 10–20% FBS, endothelial cell growth supplement (ECGS), and heparin for endothelial cells; change medium every 2 days |
| Components / Formulation | Ham's F12K with L-glutamine, sodium bicarbonate, phenol red; sterile-filtered |
| Storage Conditions | 2–8 °C, protected from light |
| Shelf Life | 12 months at 2–8 °C |
| Package Specifications | 500 mL |
| Product Form | Liquid, sterile, ready-to-use |
| Quality Control | Growth promotion verified on primary HUVEC culture; sterility and endotoxin tested per specification |
| Key Features | Kaighn's amino acid and putrescine enrichment supports primary cell growth without feeder layers in many applications |
| Purity | Sterile; endotoxin ≤0.25 EU/mL; mycoplasma-free |
| Concentration | As specified (1× ready-to-use or 100× concentrate) |
| Activity / Unit Definition | Cell growth promotion validated per lot on reference cell lines |
| Molecular Weight | Not applicable for basal media; varies for supplements |
| Source / Origin | Synthetic amino acids, vitamins, and inorganic salts; animal-origin components irradiated or tested |
| pH Range / Optimal pH | pH 7.2–7.6 at physiological CO₂ |
| Shipping Conditions | Ambient temperature for dry powder; cold packs for liquid media |
| Expiration Date / Stability | 12 months for liquid media; 24–36 months for dry powder |
| Regulatory / Compliance | Research use; GMP-grade options available; Certificate of Origin for animal-derived components |
| Compatibility | Compatible with standard mammalian cell culture incubator conditions (37 °C, 5% CO₂) |
| Recommended Buffer System | Sodium bicarbonate/CO₂ buffering system; HEPES-buffered options available |
| Application Notes / Precautions | Pre-warm to 37 °C before use; supplement with serum and antibiotics as needed; use aseptic technique |
| Batch-to-Batch Consistency | Osmolality within ±5% of reference; cell growth rate within ±15% of reference lot |
For research use only, not for clinical use.
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