- Home
- IVD
- By Technology Types
- By Diseases Types
- By Product Types
- Research
- Resource
- Distributors
- Company
| Product Name | Ham's F12 Nutrient Mixture, with L-Glutamine |
| Catalog No. | ATR-CCR-0006 |
| Description | A complex nutrient mixture developed by Dr. Richard Ham containing trace elements, polyamines, and fatty acid precursors for the clonal growth of Chinese Hamster Ovary cells and other fastidious cell types. |
| Intended Use | Clonal growth and single-cell plating of CHO cells for recombinant protein production; culture of primary rat and human fibroblasts, prostate epithelial cells, and chondrocytes; component of serum-free medium formulations. |
| Principle / Technology | F12 supplements basal medium with copper, zinc, iron, selenium trace elements; putrescine polyamine; linoleic acid; and elevated concentrations of several vitamins including biotin, B12, and lipoic acid to support low-density growth. |
| Detection Method | Clonal growth efficiency by plating at limiting dilution, recombinant protein productivity in CHO cells |
| Sample Type | CHO cells, primary fibroblasts, epithelial cells, chondrocytes, embryonic stem cells |
| Performance Range / Specifications | Clonal plating efficiency >30% for CHO cells; supports single-cell cloning |
| Sensitivity / LOD | N/A |
| Specificity | N/A |
| Reaction Conditions / Protocol | Supplement with 10% FBS for routine CHO culture; combine with DMEM 1:1 for a broader range of cell types; equilibrate in 5% CO2. |
| Components / Formulation | Ham's F12 salts, 21 amino acids (including non-essential), 14 vitamins, trace elements (CuSO4, FeSO4, ZnSO4), putrescine, linoleic acid, lipoic acid, 1.8 g/L glucose, 1.176 g/L sodium bicarbonate, 2 mM L-glutamine, phenol red |
| Storage Conditions | 2–8°C, protect from light |
| Shelf Life | 12 months |
| Package Specifications | 500 mL, 1 L |
| Product Form | Liquid, sterile-filtered |
| Quality Control | Sterility, endotoxin, pH, osmolality, CHO clonal growth promotion testing |
| Key Features | Nutrient-rich formulation; supports clonal growth; trace elements and lipids included; with L-glutamine; standard for CHO cell culture |
For research use only, not for clinical use.
|
There is no product in your cart. |
Copyright © 2026 Alta DiagnoTech. All rights reserved.
