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| Product Name | Ham's F-12 Nutrient Mix (with 1802 mg/L D-Glucose, L-Glutamine, Sodium Pyruvate and Phenol Red; without HEPES) |
| Catalog No. | RESK-0638 |
| Description | Ham's F-12 Nutrient Mix (F-12) is an optimized formulation based on Ham's F-10 medium, with increased concentrations of choline, inositol, putrescine and selected amino acids. It is suitable for serum-free culture of Chinese hamster ovary (CHO) cells and for serum-supplemented culture of other mammalian cells, including chondrocytes, rat prostate epithelial cells, rat hepatocytes and chicken embryo cells. This product is sterilized by 0.1 um filtration and can be used directly for cell culture. Contains: L-glutamine, D-glucose (1802 mg/L), sodium pyruvate and phenol red. Without: HEPES. |
| Intended Use | For serum-free culture of Chinese hamster ovary (CHO) cells and serum-supplemented culture of other mammalian cells, including chondrocytes, rat prostate epithelial cells, rat hepatocytes and chicken embryo cells. |
| Performance Range / Specifications | Complete formulation with 1802 mg/L D-glucose, L-glutamine, sodium pyruvate and phenol red; without HEPES. Sterilized by 0.1 um filtration. |
| Components / Formulation | Inorganic Salts CaCl2 (anhydrous) 33.22 mg/L Cupric sulfate (CuSO4-5H2O) 0.0025 mg/L FeSO4-7H2O 0.834 mg/L MgCl2 (anhydrous) 57.22 mg/L KCl 223.6 mg/L NaHCO3 1176 mg/L NaCl 7599 mg/L NaH2PO4 (anhydrous) 142 mg/L ZnSO4-7H2O 0.863 mg/L Amino Acids Glycine 7.5 mg/L L-Alanine 8.9 mg/L L-Arginine hydrochloride 211 mg/L L-Asparagine-H2O 15.01 mg/L L-Aspartic acid 13.3 mg/L L-Cysteine hydrochloride-H2O 35.12 mg/L L-Glutamic Acid 14.7 mg/L L-Glutamine 146 mg/L L-Histidine hydrochloride-H2O 21 mg/L L-Isoleucine 4 mg/L L-Leucine 13.1 mg/L L-Lysine hydrochloride 36.5 mg/L L-Methionine 4.5 mg/L L-Phenylalanine 5 mg/L L-Proline 34.5 mg/L L-Serine 10.5 mg/L L-Threonine 11.9 mg/L L-Tryptophan 2.04 mg/L L-Tyrosine disodium salt dihydrate 7.81 mg/L L-Valine 11.7 mg/L Vitamins Biotin 0.0073 mg/L Choline chloride 14 mg/L D-Calcium pantothenate 0.5 mg/L Folic Acid 1.3 mg/L Niacinamide 0.036 mg/L Pyridoxine-HCl 0.06 mg/L Riboflavin 0.037 mg/L Thiamine-HCl 0.3 mg/L Vitamin B12 1.4 mg/L i-Inositol 18 mg/L Other Components D-Glucose 1802 mg/L Hypoxanthine Na 4.77 mg/L Linoleic Acid 0.084 mg/L Lipoic Acid 0.21 mg/L Phenol Red 1.2 mg/L Putrescine 2HCl 0.161 mg/L Sodium Pyruvate 110 mg/L Thymidine 0.7 mg/L |
| Storage Conditions | Store at 2-8 °C, protected from light. |
| Shelf Life | 1 year when stored at 2-8 °C, protected from light. |
| Package Specifications | 500 mL |
| Product Form | Liquid, 1x ready-to-use cell culture medium |
| Quality Control | Sterilized by 0.1 um filtration. |
| Regulatory / Compliance | For research use only. |
| Application Notes / Precautions | This formulation contains no HEPES; use in a CO2-equilibrated incubator as appropriate for the target cells. Protect from light during storage. |
For research use only, not for clinical use.
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