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| Product Name | HaeIII Rapid Restriction Endonuclease (10 U/uL) |
| Catalog No. | IVDRM-0427 |
| Description | Cutting the CpG rich blunt site 5 prime GG^CC 3 prime, this rapid recombinant endonuclease supports gene cloning, recombinant vector construction and plasmid linearisation with digestion of plasmid DNA, PCR products and genomic DNA in 5 to 15 minutes. It is supplied at 10 U/uL and performs in the unified rapid series buffer at 37°C, with heat inactivation at 80°C for 20 minutes. Dam, Dcm, CpG, EcoKI and EcoBI methylation all leave activity unchanged, a notable tolerance among CpG cutting enzymes, and BsuRI, BshFI, BspANI and BsnI are documented isoschizomers. |
| Intended Use | Molecular cloning, vector construction and plasmid linearisation with rapid blunt digestion |
| Principle / Technology | Site specific blunt cleavage at 5 prime GG^CC 3 prime within 5 to 15 minutes at 37°C |
| Specificity | Unaffected by Dam, Dcm, CpG, EcoKI and EcoBI methylation; isoschizomers BsuRI, BshFI, BspANI and BsnI |
| Reaction Conditions / Protocol | Digest in 1x rapid restriction buffer at 37°C for 5 to 15 minutes; heat inactivate at 80°C for 20 minutes |
| Storage Conditions | -25 to -15°C |
| Shelf Life | 2 years |
| Package Specifications | 300 T |
| Key Features | Broad methylation tolerance; single shared buffer across the rapid series |
| Concentration | 10 U/uL |
| Readouts / Downstream Assays | Agarose gel analysis of digested DNA |
For in vitro diagnostic use.
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