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| Product Name | Green Nucleic Acid Gel Stain (10,000× in Water) |
| Catalog No. | IVDRM-0107 |
| Description | Green Nucleic Acid Gel Stain is a unique oily macromolecule that cannot penetrate cell membranes and is not volatile or sublimable, so it cannot be inhaled. Ames test results show that the stain is completely non-mutagenic at gel-staining concentrations, making it a safe, non-toxic nucleic acid dye compared with the strongly carcinogenic EB. It is suitable for staining nucleic acids of all fragment sizes and has far less effect on nucleic acid mobility than SYBR Green I. The stain is compatible with agarose gel preparation using microwave or other heating methods, is extremely stable in acidic or alkaline buffers at room temperature, and offers strong light resistance. It can be used to stain dsDNA, ssDNA, and RNA in agarose and polyacrylamide gel electrophoresis by either in-gel (pre-cast) staining or post-electrophoresis soaking. Sharing the spectral properties of SYBR Green I, bands can be observed directly on visible-light (blue-light) gel transilluminators and blue-light gel cutters, and the stain is also suitable for UV gel imaging transilluminators using 488 nm excitation. |
| Intended Use | Staining of dsDNA, ssDNA, and RNA of all fragment sizes in agarose and polyacrylamide gel electrophoresis, either before (in-gel) or after (post-staining) electrophoresis. |
| Principle / Technology | A safe, non-mutagenic fluorescent nucleic acid dye and EB alternative with spectral properties matching SYBR Green I; the fluorescent dye binds nucleic acids and emits detectable signal under blue-light or 488 nm excitation. |
| Detection Method | Excitation at 471 nm; observe on a laser imager, visible-light (blue-light) gel transilluminator, or blue-light gel cutter; also compatible with UV gel imaging transilluminators using 488 nm excitation. |
| Sample Type | dsDNA, ssDNA, and RNA of various fragment sizes separated in agarose or polyacrylamide gels. |
| Performance Range / Specifications | Supplied at 10,000× concentration in water Excitation wavelength: 471 nm (also suitable for 488 nm excitation) Minimal effect on nucleic acid mobility, far less than SYBR Green I Extremely stable in acidic or alkaline buffers at room temperature; strong light resistance Stable at room temperature with a 3-year shelf life Suitable for staining agarose or polyacrylamide gels before or after electrophoresis |
| Reaction Conditions / Protocol | In-gel staining: add the 10,000× stain to the molten gel solution before casting (compatible with microwave or other heating-based gel preparation). Post-staining: soak the gel in diluted staining solution after electrophoresis. |
| Components / Formulation | Green Nucleic Acid Gel Stain (10,000× in Water) |
| Storage Conditions | Store at room temperature protected from light. |
| Shelf Life | 3 years. |
| Package Specifications | 500 μL |
| Product Form | Liquid (10,000× aqueous solution) |
| Quality Control | Ames test confirms the stain is completely non-mutagenic at gel-staining concentrations. |
| Concentration | 10,000× in water |
| pH Range / Optimal pH | Extremely stable in acidic or alkaline buffers at room temperature. |
| Expiration Date / Stability | Stable at room temperature for 3 years when protected from light; strong light resistance. |
| Regulatory / Compliance | Safe and non-toxic: completely non-mutagenic at gel-staining concentrations (Ames test), providing a safe alternative to the strongly carcinogenic ethidium bromide. |
| Compatibility | Compatible with gel recovery of target bands after electrophoresis. Observable on laser imagers, visible-light (blue-light) transilluminators, and blue-light gel cutters; also suitable for UV gel imaging systems with 488 nm excitation. |
| Application Notes / Precautions | Target bands can be recovered from the gel after electrophoresis for downstream applications. The stain has far less effect on nucleic acid migration than SYBR Green I. Unlike UV-excited red dyes (excitation ~304 nm, observed on UV imagers), this green dye is excited at 471 nm and is observed on laser or visible-light imagers. The stain remains extremely stable in acidic or alkaline buffers at room temperature and is strongly resistant to light. |
For research use only, not for clinical use.
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