GMP-Grade T7 RNA Polymerase (250 U/uL)
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GMP-Grade T7 RNA Polymerase (250 U/uL)

Cat.No: IVDRM-0355 Datasheet

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Product Details Related Products
Product Name GMP-Grade T7 RNA Polymerase (250 U/uL)
Catalog No. IVDRM-0355
Description Derived from recombinant expression in E. coli, this high-concentration T7 RNA polymerase copyies any double-stranded DNA carrying a T7 promoter into RNA in the presence of NTPs. Blunt-ended or 5-prime overhanging linear DNA from plasmids or PCR reactions is accepted without further processing, supporting transcripts of 20-10,000 nt and typical yields of 100-200 ug RNA per ug of template. Endonuclease, exonuclease and RNase activities are below detection, the enzyme is produced under GMP regulations, and the liquid is filled at 250 U/uL.
Intended Use Concentrated enzyme format for in vitro transcription in mRNA vaccine and drug development; synthesis of RNA from linear plasmid or PCR-amplified templates.
Specificity Yield of 100-200 ug RNA per ug template; transcript range 20-10,000 nt; free of endonuclease, exonuclease and RNase
Storage Conditions -25 °C to -15 °C
Shelf Life 12 months
Package Specifications 10 KU, 100 KU, 2.5 MU and 25 MU packs
Product Form Liquid
Concentration 250 U/uL
Source / Origin Recombinant T7 RNA polymerase expressed in E. coli
Regulatory / Compliance GMP grade
Model Type Phage T7 RNA-dependent RNA polymerase, GMP grade, high concentration

For in vitro diagnostic use.

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