- Home
- IVD
- By Technology Types
- By Diseases Types
- By Product Types
- Research
- Resource
- Distributors
- Company
| Product Name | GMP-Grade Recombinant Broad-Spectrum Endonuclease for Host Residual Nucleic Acid Removal (250-300 U/uL) |
| Catalog No. | IVDRM-0369 |
| Description | This GMP-grade non-specific endonuclease, derived from Serratia marcescens and expressed in E. coli under GMP conditions, cleaves nucleic acids at any inter-nucleotide position into 2 to 5 base 5-monophosphate oligonucleotides. It degrades all forms of DNA and RNA even in the presence of 6 M urea, 0.1 M guanidine hydrochloride, 0.4% Triton X-100, 0.1% SDS, 1 mM EDTA or 1 mM PMSF. Beyond reducing viscosity in protein purification, it serves as a host residual nucleic acid removal reagent in virus purification, vaccine production and protein or polysaccharide manufacturing, lowering residual nucleic acid to picogram levels to improve product efficacy and safety, and it prevents PBMC clumping in cell therapy workflows. |
| Intended Use | Host residual nucleic acid removal in virus purification, vaccine production and protein or polysaccharide manufacturing; viscosity reduction in protein purification; PBMC clumping prevention |
| Principle / Technology | Non-specific endonucleolytic cleavage digesting DNA and RNA into short 5-monophosphate oligonucleotides |
| Sample Type | Cell supernatants; cell lysates; virus; vaccines; proteins; polysaccharides; peripheral blood mononuclear cells |
| Storage Conditions | -15 to -25°C; filter sterilise after more than one week at 4°C following opening |
| Shelf Life | 2 years |
| Package Specifications | 25 KU; 50 KU; 100 KU; 1 MU (1000 KU); 5 MU (5000 KU) |
| Key Features | GMP grade; drug master file filed; protease activity negative; sterility negative; endotoxin <0.25 EU per 1000 U; host protein <10 ppm |
| Purity | >=99% (SDS-PAGE) |
| Concentration | 250-300 U/uL |
| Activity / Unit Definition | Specific activity >=1.5E6 U/mg; one unit digests 37 ug salmon sperm DNA in 30 minutes at 37°C and pH 8.0 with a 1.0 change in A260 in a 2.625 mL reaction |
| Molecular Weight | 26.5 kDa |
| Source / Origin | Serratia marcescens derived, expressed in E. coli under GMP conditions |
| Shipping Conditions | Dry ice |
| Recommended Buffer System | 20 mM Tris-HCl pH 8.0, 2 mM MgCl2, 20 mM NaCl, 50% glycerol |
For in vitro diagnostic use.
|
There is no product in your cart. |
Copyright © 2026 Alta DiagnoTech. All rights reserved.
