GMP-Grade Low-dsRNA T7 RNA Polymerase (250 U/uL)
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GMP-Grade Low-dsRNA T7 RNA Polymerase (250 U/uL)

Cat.No: IVDRM-0358 Datasheet

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Product Details Related Products
Product Name GMP-Grade Low-dsRNA T7 RNA Polymerase (250 U/uL)
Catalog No. IVDRM-0358
Description Engineered from the wild-type phage T7 enzyme through directed mutation, this polymerase represses the double-stranded RNA impurity generated during in vitro transcription by at least an order of magnitude while preserving high synthetic output. Reactions reach yields stable above 9 mg/mL, cap at rates exceeding 99% and work with cap analog inputs as low as 2.5 mM, easing the burden on downstream purification. Manufactured under GMP regulations with drug master file filing and ISO 13485 quality management, the liquid enzyme is offered at 250 U/uL for mRNA vaccine and drug development.
Intended Use GMP-suitable in vitro transcription of mRNA where low immunostimulatory dsRNA content and high capping efficiency are required.
Specificity dsRNA content reduced at least 10-fold; capping rate above 99%; cap analog input down to 2.5 mM; yield stable above 9 mg/mL
Storage Conditions -25 °C to -15 °C
Shelf Life 12 months
Package Specifications 10 KU, 100 KU, 25 MU and 100 MU packs
Product Form Liquid
Concentration 250 U/uL
Source / Origin Engineered recombinant T7 RNA polymerase expressed in E. coli
Regulatory / Compliance GMP grade; drug master file filed; ISO 13485 quality system
Model Type Engineered low-dsRNA T7 RNA polymerase, GMP grade

For in vitro diagnostic use.

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