| Product Name | Glutathione Reductase (GR) Activity Assay Reagent (Microplate Reader) |
| Catalog No. | ETR-HMM-0014 |
| Description | Glutathione reductase (GR) is the primary flavoprotein enzyme responsible for maintaining reduced glutathione (GSH) levels in cells. As one of the key enzymes in the glutathione redox cycle, it catalyzes the reduction of oxidized glutathione (GSSG) to reduced glutathione (GSH), thereby helping to maintain the GSH/GSSG ratio in the body. It plays a significant role in oxidative stress responses and the ascorbic acid-glutathione cycle pathway. |
| Application | GR catalyzes the reduction of GSSG to GSH using NADPH, while NADPH is oxidized to NADP+. NADPH has a characteristic absorption peak at 340 nm, and changes in absorbance can be used to characterize the activity of glutathione reductase. |
| Applicable Instruments | Microplate Reader |
| Number of Testable Samples | 100 Samples |
| Matching | 96-well plate |
| Detection Time | 5 h (100 Samples) |
| Detection Method | NADPH method |
| Spectral Parameters | 340 nm |
| Signal Response | Decremental |
| Notes | All processes, including the preparation of crude enzyme solution, should be performed on ice. It is recommended to complete the measurement on the same day after sample processing, and avoid repeated freezing and thawing of the crude enzyme solution; Reagent 1 contains a certain concentration of protein (approximately 0.1 mg/mL). When measuring the protein concentration of the sample, subtract the protein content of the reagent itself; Maintain a constant temperature of 37°C (for mammals) or 25°C (for other species) during the reaction; It is recommended to add an appropriate amount of distilled water at 37°C (for mammals) or 25°C (for other species) to a beaker, place this beaker in a water bath maintained at 37°C (for mammals) or 25°C (for other species), and place the cuvette containing the reaction solution in this beaker during the reaction process; Take readings accurately at 10 seconds and 190 seconds to ensure the accuracy of the experimental results; If using a 96-well UV plate for measurement, use a multichannel pipette and perform the measurements in batches to ensure consistent reaction times between groups; |
For research use only, not for clinical use.
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