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| Product Name | Xanthine Oxidase Activity Fluorometric Assay Kit |
| Catalog No. | ETR-HMM-0077 |
| Description | A fluorometric assay kit for measuring xanthine oxidase enzyme activity in biological samples. The assay employs a probe that reacts with hydrogen peroxide, a by-product of the xanthine oxidase-catalyzed conversion of hypoxanthine to xanthine and subsequently to uric acid, to generate a fluorescent signal proportional to enzyme activity. The kit includes a specific inhibitor to distinguish xanthine oxidase activity from background and other peroxide-generating systems. |
| Intended Use | Quantitative measurement of xanthine oxidase activity in serum, tissue homogenates, and cell lysates for studying purine metabolism and oxidative stress. |
| Principle / Technology | Enzyme-coupled fluorometric detection of xanthine oxidase-generated hydrogen peroxide |
| Detection Method | Fluorescence (excitation 535 nm, emission 587 nm) |
| Sample Type | Serum, plasma, tissue homogenates, cell lysates, purified enzyme preparations |
| Performance Range / Specifications | Linear detection range: 0.5–50 mU/mL; reaction time: 30 min |
| Sensitivity / LOD | Detection limit: 0.2 mU/mL |
| Specificity | Specific to xanthine oxidase; minimal interference from xanthine dehydrogenase or aldehyde oxidase |
| Reaction Conditions / Protocol | Mix sample with reaction cocktail (hypoxanthine substrate, fluorescent probe, HRP); incubate 30 min at 37 °C protected from light; read fluorescence |
| Components / Formulation | Hypoxanthine substrate (100×), fluorescent probe (200×), HRP enzyme, xanthine oxidase positive control, assay buffer, allopurinol inhibitor control, H₂O₂ standard |
| Storage Conditions | –20 °C for probe and enzyme; 2–8 °C for buffer and substrate |
| Shelf Life | 6 months at recommended temperature |
| Package Specifications | 100 tests |
| Product Form | Liquid reagents |
| Quality Control | Functional validation with purified xanthine oxidase standard; intra-assay CV ≤8% |
| Key Features | Inhibitor control well enables quantification of xanthine oxidase-specific activity separate from background |
| Purity | Reagent grade; substrate purity ≥98% by HPLC where applicable |
| Concentration | As specified per kit; calibrator and substrate concentrations traceable to reference |
| Activity / Unit Definition | One unit defined as the amount of enzyme that catalyzes conversion of 1 µmol substrate per minute under specified conditions |
| Molecular Weight | Varies by target enzyme as documented |
| Source / Origin | Recombinant or purified from natural sources; synthetic chromogenic/fluorogenic substrates |
| pH Range / Optimal pH | pH 6.0–9.0 depending on specific enzyme assay |
| Shipping Conditions | Cold packs for enzyme components; ambient for buffers |
| Expiration Date / Stability | 6–12 months at recommended storage temperature |
| Regulatory / Compliance | Research use; manufactured under ISO 9001 quality system |
| Compatibility | Compatible with standard microplate readers (absorbance, fluorescence, luminescence modes) |
| Recommended Buffer System | Tris or phosphate buffer optimized per enzyme assay |
| Application Notes / Precautions | Include positive control and inhibitor control in each assay; read within linear range; protect light-sensitive substrates |
| Batch-to-Batch Consistency | Enzyme activity and standard curve linearity within ±15% of reference lot |
For research use only, not for clinical use.
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