- Home
- IVD
- By Technology Types
- By Diseases Types
- By Product Types
- Research
- Resource
- Distributors
- Company
| Product Name | Angiotensin-Converting Enzyme (ACE) Activity Colorimetric Assay Kit |
| Catalog No. | ETR-HMM-0078 |
| Description | A colorimetric assay kit for determining angiotensin-converting enzyme activity in biological fluids using a synthetic peptide substrate. ACE cleaves the substrate to release a chromogenic product that is quantified by absorbance measurement. The assay is configured for serum and plasma specimens as well as purified protein preparations, and includes a selective ACE inhibitor control to confirm signal specificity. |
| Intended Use | Determination of ACE enzymatic activity in serum and plasma for clinical and pharmacological research applications. |
| Principle / Technology | Enzymatic cleavage of a synthetic chromogenic peptide substrate specific to ACE |
| Detection Method | Colorimetric (absorbance at 405 nm) |
| Sample Type | Human serum, EDTA or heparin plasma; avoid hemolyzed samples |
| Performance Range / Specifications | Linear range: 5–150 U/L; incubation: 30 min at 37 °C |
| Sensitivity / LOD | Lower detection limit: 3 U/L |
| Specificity | Substrate is specifically cleaved by ACE; inhibitor control (captopril-based) blocks ≥95% of signal |
| Reaction Conditions / Protocol | Sample volume: 20 µL; add substrate and incubate 30 min at 37 °C; stop reaction and read at 405 nm |
| Components / Formulation | ACE substrate solution, ACE inhibitor solution (captopril), reaction buffer, stop solution, ACE calibrator |
| Storage Conditions | 2–8 °C for all components |
| Shelf Life | 12 months |
| Package Specifications | 100 tests |
| Product Form | Liquid, ready-to-use reagents |
| Quality Control | Calibrator value assigned against NIST-traceable reference material; within-run precision CV ≤6% |
| Key Features | Inhibitor control included to confirm assay specificity within each run |
| Purity | Reagent grade; substrate purity ≥98% by HPLC where applicable |
| Concentration | As specified per kit; calibrator and substrate concentrations traceable to reference |
| Activity / Unit Definition | One unit defined as the amount of enzyme that catalyzes conversion of 1 µmol substrate per minute under specified conditions |
| Molecular Weight | Varies by target enzyme as documented |
| Source / Origin | Recombinant or purified from natural sources; synthetic chromogenic/fluorogenic substrates |
| pH Range / Optimal pH | pH 6.0–9.0 depending on specific enzyme assay |
| Shipping Conditions | Cold packs for enzyme components; ambient for buffers |
| Expiration Date / Stability | 6–12 months at recommended storage temperature |
| Regulatory / Compliance | Research use; manufactured under ISO 9001 quality system |
| Compatibility | Compatible with standard microplate readers (absorbance, fluorescence, luminescence modes) |
| Recommended Buffer System | Tris or phosphate buffer optimized per enzyme assay |
| Application Notes / Precautions | Include positive control and inhibitor control in each assay; read within linear range; protect light-sensitive substrates |
| Batch-to-Batch Consistency | Enzyme activity and standard curve linearity within ±15% of reference lot |
For research use only, not for clinical use.
|
There is no product in your cart. |
Copyright © 2026 Alta DiagnoTech. All rights reserved.
