Lipase (LPS) Assay Reagent (Colorimetric Method)
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Lipase (LPS) Assay Reagent (Colorimetric Method)

Cat.No: ETR-HMM-0039 Datasheet

Specification Quantities

48T:
- +
Product Details Related Products
Product Name Lipase (LPS) Assay Reagent (Colorimetric Method)
Catalog No. ETR-HMM-0039
Description Human lipase primarily originates from the pancreas. Elevated serum LPS is commonly seen in acute pancreatitis and pancreatic cancer, and occasionally in chronic pancreatitis. In acute pancreatitis, serum amylase levels rise for a short period of time, while serum LPS levels remain elevated for 10-15 days. In mumps, LPS is usually within the normal range, so LPS is more specific for research on acute pancreatitis. The basic constituent unit of lipase is only amino acids, usually with only one polypeptide chain. Its catalytic activity is determined solely by its protein structure. Lipase is an enzyme that breaks down fat, and serum lipase measurement is mainly used in the research of acute pancreatitis. Triglycerides and water-based latex have a milky appearance due to the absorption and scattering of incident light by their micelles. Under the action of lipase, the substrate undergoes hydrolysis, causing the micelles to split, thereby reducing scattered light or turbidity. The rate of reduction is related to lipid content and enzyme activity.
Application This product uses a colorimetric method to measure LPS activity.
Applicable Instruments Visible Spectrophotometer
Number of Testable Samples 48 Samples
Detection Time 20 min (20-40 Samples)
Detection Method Colorimetric method
Spectral Parameters 420 nm
Standard Linear Range 5.0-2000 U/L
Detection Limit 5.0 U/L
Inter-batch Variation CV % 7.44
Intra-batch Variation CV % 3.5
Coefficient of Variation CV % 1.7
Recovery Rate % 102
Storage 2-8°C
Shelf Life 6 months

For research use only, not for clinical use.

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