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| Product Name | GlutaMAX Supplement, 100x, Stable Dipeptide Glutamine |
| Catalog No. | ATR-CCR-0025 |
| Description | A sterile 100-fold concentrated solution of the dipeptide L-alanyl-L-glutamine as a stable alternative to L-glutamine, providing equal nutritional benefit without the accumulation of neurotoxic ammonia in long-term cell culture. |
| Intended Use | Replacement for L-glutamine in cell culture media, providing a stable glutamine source that does not degrade spontaneously and accumulates less cytotoxic ammonia, suitable for extended culture and high-density bioprocessing. |
| Principle / Technology | The dipeptide L-alanyl-L-glutamine is resistant to spontaneous degradation and thermal decomposition during storage; cells sequentially cleave the dipeptide through cell-surface aminopeptidases, releasing glutamine on demand at the cell membrane. |
| Detection Method | Ammonia concentration measurement in culture supernatant, cell growth comparison with free L-glutamine |
| Sample Type | All mammalian cell culture types, particularly high-density cultures and cells sensitive to ammonia |
| Performance Range / Specifications | 200 mM dipeptide at 100x concentration; ammonia accumulation reduced >50% compared to equivalent free glutamine over 5-day culture |
| Sensitivity / LOD | N/A |
| Specificity | N/A |
| Reaction Conditions / Protocol | Add 5 mL per 500 mL medium (1:100 dilution to 2 mM); replaces L-glutamine at equimolar concentration. |
| Components / Formulation | L-alanyl-L-glutamine dipeptide 200 mM in 0.85% NaCl, sterile-filtered |
| Storage Conditions | 15–30°C, protect from light |
| Shelf Life | 24 months |
| Package Specifications | 100 mL, 500 mL |
| Product Form | Liquid concentrate (100x), sterile |
| Quality Control | Concentration verification by HPLC, sterility, endotoxin, ammonia generation comparison versus free glutamine, cell growth promotion |
| Key Features | Thermally stable at 2–8°C; reduced ammonia accumulation; room temperature storage; sterile 100x concentrate; ideal for extended cultures and bioprocessing |
For research use only, not for clinical use.
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