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| Product Name | Gentle Cell Dissociation Solution (Ready-to-Use) |
| Catalog No. | RESK-0261 |
| Description | This product is a natural enzyme mixture with proteolytic and collagenolytic activities, combining the effects of both protease and collagenase. It is a ready-to-use (sterile) cell dissociation solution containing no animal- or bacterial-derived components, suitable for all applications of conventional cell dissociation reagents and directly applicable to cell culture experiments. Because it is more effective than conventional mammalian protease and collagenase preparations, its formulation concentration is much lower, making it less toxic, gentler, and more effective. Key advantages compared with traditional dissociation reagents: 1) Suitable whenever gentle and efficient detachment of any adherent cell line is required. 2) Works very well on embryonic and neuronal stem cells; monolayer stem cells can be grown after passaging with this solution. 3) Preserves most epitopes for subsequent flow cytometry analysis. 4) No neutralization is required when passaging adherent cells. Adding more medium after cell detachment dilutes the solution so that it no longer dissociates cells. Adherent cell detachment is achieved within a few minutes, with no washing or neutralization needed, saving passaging time. 5) No aliquoting required; one bottle remains stable in the refrigerator for 2 months. 6) Suitable for dissociation of the vast majority of primary cells and mammalian cell lines, as well as insect cells. 7) Gently and effectively dissociates stem cells (hESCs and mESCs), giving higher recovery rates after cryopreservation. 8) Gentle cell dissociation increases cell yield and viability, enhances cell attachment efficiency, and improves cell morphology and growth characteristics. This solution is commonly used to detach cells from routine tissue culture vessels and adhesion cultureware, and is suitable for tissue dissociation and cell isolation. Dissociated cells retain intact surface antigens, and the solution contains no animal- or bacterial-derived components, meeting the requirements of downstream experiments such as cell surface labeling, virus growth analysis, flow cytometry, and bioreactor-related assays. |
| Intended Use | Gentle dissociation/detachment of adherent cells from tissue culture vessels; tissue dissociation and cell isolation for cell culture, stem cell passaging, flow cytometry sample preparation, and cell surface marker analysis. |
| Principle / Technology | A natural enzyme mixture with proteolytic and collagenolytic activities that gently cleaves cell-substrate and cell-cell attachments; effective at a much lower formulation concentration than conventional protease/collagenase preparations, resulting in lower toxicity and better preservation of cell surface epitopes. |
| Sample Type | Adherent mammalian cell lines, primary cells, embryonic stem cells (hESCs and mESCs), neuronal stem cells, and insect cells. |
| Specificity | Preserves most cell surface epitopes, compatible with subsequent flow cytometry analysis. |
| Reaction Conditions / Protocol | 1. Thaw the solution at 4 °C or on ice, then aliquot for storage if needed. Note: Do not thaw at 37 °C, as this will impair enzyme activity. 2. Rinse cells once with 1x Dulbecco's PBS (without calcium and magnesium). 3. Under sterile conditions, add 10 mL of dissociation solution per 75 cm2 surface area to cover the adherent cells. 4. Return the vessel to a 37 °C incubator and incubate for 5-10 min. Note: Incubation time can be adjusted. Most cells tolerate exposure for up to 1 hour without adverse effects. 5. After dissociation, gently tap the dish to detach cells from the bottom, then perform cell counting and passaging as usual. Note: No additional washing or enzyme inactivation step is required. |
| Components / Formulation | Ready-to-use sterile solution; a natural enzyme mixture with proteolytic and collagenolytic activities; free of animal- and bacterial-derived components. |
| Storage Conditions | Store at -25 to -15 °C for long-term storage (2-year shelf life). Store at 2-8 °C after thawing (stable for 2 months). Do not store at room temperature. Alternatively, aliquot into single-use portions and freeze at -80 °C (stable for at least 6 months). |
| Shelf Life | 2 years at -25 to -15 °C; 2 months at 2-8 °C (after thawing); at least 6 months at -80 °C (single-use aliquots). |
| Package Specifications | 100 mL |
| Product Form | Ready-to-use sterile liquid. |
| Source / Origin | Natural enzyme mixture; contains no animal- or bacterial-derived components. |
| Expiration Date / Stability | Stable for at least 2 months at 2-8 °C after thawing; stable for at least 6 months at -80 °C when aliquoted; 2-year shelf life at -25 to -15 °C. |
| Regulatory / Compliance | For research use only. Not for clinical diagnostic use. |
| Compatibility | Compatible with cell culture, stem cell passaging, flow cytometry, virus growth analysis, cell surface labeling, and bioreactor-related assays. |
| Recommended Buffer System | Rinse cells with 1x Dulbecco's PBS (without calcium and magnesium) before dissociation. |
| Application Notes / Precautions | Do not thaw at 37 °C; thaw at 4 °C or on ice. Do not store at room temperature. No neutralization or washing is required after dissociation; adding fresh medium dilutes the enzyme activity. Most cells tolerate up to 1 hour of exposure without adverse effects. |
For research use only, not for clinical use.
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