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| Product Name | Gentle Cell Dissociation Solution |
| Catalog No. | RESK-0237 |
| Description | This gentle cell dissociation solution combines proteolytic and collagenase activities and is a perfect replacement for conventional trypsin/EDTA solutions for detaching cells from standard and adhesion culture vessels. It is suitable for tissue dissociation and cell separation; because dissociated cells retain intact surface antigens and the formulation contains no animal- or bacterial-derived components, it meets the requirements of downstream applications such as cell surface marker analysis, virus growth analysis, flow cytometry, and bioreactor-related assays. The product is a ready-to-use sterile solution formulated in Dulbecco's PBS (containing 0.5 mM EDTA and 3 mg/L phenol red) with a specific activity of >=200 u/mL, and can be used directly for cell culture. Digestion is gentle and effective: cells are detached within minutes without washing or neutralization steps, improving cell yield, viability, attachment efficiency, morphology, and growth characteristics. It gently and effectively dissociates stem cells (hESCs and mESCs), yielding higher recovery rates after cryopreservation. |
| Intended Use | For gentle dissociation and passaging of most primary cells, mammalian cell lines, and insect cells; especially suitable for stem cells and primary cells, and for workflows involving cell surface marker analysis, virus growth analysis, flow cytometry, and bioreactor-related assays. For research use only. |
| Principle / Technology | A blend of proteolytic and collagenase activities gently cleaves cell-substrate and cell-cell contacts, enabling rapid detachment of adherent cells while preserving the integrity of cell surface antigens and cell viability. The gentle action eliminates the need for washing or enzyme neutralization, saving passaging time. |
| Sample Type | Adherent cultured cells (primary cells, mammalian cell lines, insect cells, stem cells) and tissues for dissociation |
| Performance Range / Specifications | Specific activity: >=200 u/mL Ready-to-use sterile solution Suitable for most primary cells, mammalian cell lines, and insect cells Gently and effectively dissociates stem cells (hESCs and mESCs); higher recovery after cryopreservation Detaches adherent cells within minutes; no washing or neutralization required Gentle digestion improves cell yield, viability, attachment efficiency, morphology, and growth characteristics |
| Reaction Conditions / Protocol | 1. Thaw the solution at 4 °C or in an ice bath, then aliquot for storage. Note: Do NOT thaw at 37 °C, as this will impair enzyme activity. 2. Rinse cells once with calcium- and magnesium-free 1x Dulbecco's PBS. 3. Using aseptic technique, add 10 mL of the solution per 75 cm2 of surface area to cover the adherent cells. 4. Return the vessel to a 37 °C incubator and digest for 5-10 min. Note: Digestion time can be adjusted as needed; most cells tolerate exposure for up to 1 hour without adverse effects. 5. After digestion, gently tap the culture vessel to detach the cells, then count and passage according to routine procedures. Note: No additional washing or enzyme inactivation steps are required. |
| Components / Formulation | Ready-to-use sterile solution in Dulbecco's PBS containing 0.5 mM EDTA and 3 mg/L phenol red; contains proteolytic and collagenase enzyme activities; free of animal- or bacterial-derived components. |
| Storage Conditions | Store at -20 °C upon receipt (shelf life 2 years). Alternatively, store at 2-8 °C (shelf life 2 months). |
| Shelf Life | 2 years at -20 °C; 2 months at 2-8 °C. |
| Package Specifications | 100 mL |
| Product Form | Ready-to-use sterile liquid |
| Quality Control | Sterile; specific activity >=200 u/mL. |
| Purity | Contains no animal- or bacterial-derived components. |
| Activity / Unit Definition | Specific activity >=200 u/mL. |
| Source / Origin | Non-animal, non-bacterial origin (free of animal- or bacterial-derived components) |
| Shipping Conditions | Shipped with ice packs. |
| Expiration Date / Stability | 2 years at -20 °C; 2 months at 2-8 °C. Do not thaw at 37 °C; thaw at 4 °C or in an ice bath to preserve enzyme activity. |
| Regulatory / Compliance | For research use only. |
| Compatibility | Compatible with most primary cells, mammalian cell lines, insect cells, and stem cells (hESCs, mESCs); suitable for downstream cell surface marker analysis, virus growth analysis, flow cytometry, and bioreactor applications. |
| Recommended Buffer System | Formulated in Dulbecco's PBS (with 0.5 mM EDTA and 3 mg/L phenol red). Rinse cells with calcium- and magnesium-free 1x Dulbecco's PBS before use. |
| Application Notes / Precautions | Do not thaw at 37 °C. Most cells remain unaffected after up to 1 hour of exposure. No washing or neutralization is required after dissociation. |
| Heat Inactivation | No washing or enzyme inactivation/neutralization steps are required after dissociation. |
For research use only, not for clinical use.
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