- Home
- IVD
- By Technology Types
- By Diseases Types
- By Product Types
- Research
- Resource
- Distributors
- Company
| Product Name | Gentamicin-Amphotericin B Solution (100×), Antimycotic-Antibiotic |
| Catalog No. | ATR-CCR-0035 |
| Description | A combined antibiotic-antimycotic solution at 100× concentration containing gentamicin and amphotericin B for the prevention of bacterial and fungal contamination in cell culture. Gentamicin is an aminoglycoside antibiotic effective against a broad spectrum of gram-negative and gram-positive bacteria, while amphotericin B is a polyene antimycotic active against yeast and fungi. This combination provides comprehensive antimicrobial protection for long-term cell culture experiments. |
| Intended Use | Broad-spectrum antimicrobial protection for cell culture against both bacterial and fungal contamination. |
| Principle / Technology | Gentamicin suppression of bacterial protein synthesis combined with amphotericin B disruption of fungal membrane integrity |
| Detection Method | Visual inspection of culture for turbidity; absence of contamination confirmed by sterility testing |
| Sample Type | All mammalian cell culture systems, particularly primary cell cultures and long-term experiments at elevated contamination risk |
| Performance Range / Specifications | Working concentration: 1× (1:100 dilution); gentamicin at 50 µg/mL, amphotericin B at 2.5 µg/mL final |
| Sensitivity / LOD | Bactericidal against common laboratory contaminants including Staphylococcus, Pseudomonas, and E. coli strains |
| Specificity | Gentamicin: gram-positive and gram-negative bacteria; amphotericin B: Candida, Aspergillus, and Saccharomyces species |
| Reaction Conditions / Protocol | Add 1 mL per 100 mL culture medium; use aseptic technique; note that amphotericin B is toxic to some cell types at concentrations above 5 µg/mL |
| Components / Formulation | Gentamicin (5 mg/mL) and amphotericin B (250 µg/mL) in sterile water, 0.22 µm filtered |
| Storage Conditions | –20 °C for long-term; 2–8 °C for up to 4 weeks after thawing; protect from light |
| Shelf Life | 24 months at –20 °C |
| Package Specifications | 20 mL (sufficient for 2 L medium) |
| Product Form | Liquid, sterile, frozen or refrigerated |
| Quality Control | Sterility verified; antimicrobial potency confirmed by minimum inhibitory concentration testing against reference bacterial and fungal strains |
| Key Features | Gentamicin complements penicillin-streptomycin coverage with enhanced gram-negative activity for comprehensive antibacterial protection |
| Purity | Sterile; endotoxin ≤0.25 EU/mL; mycoplasma-free |
| Concentration | As specified (1× ready-to-use or 100× concentrate) |
| Activity / Unit Definition | Cell growth promotion validated per lot on reference cell lines |
| Molecular Weight | Not applicable for basal media; varies for supplements |
| Source / Origin | Synthetic amino acids, vitamins, and inorganic salts; animal-origin components irradiated or tested |
| pH Range / Optimal pH | pH 7.2–7.6 at physiological CO₂ |
| Shipping Conditions | Ambient temperature for dry powder; cold packs for liquid media |
| Expiration Date / Stability | 12 months for liquid media; 24–36 months for dry powder |
| Regulatory / Compliance | Research use; GMP-grade options available; Certificate of Origin for animal-derived components |
| Compatibility | Compatible with standard mammalian cell culture incubator conditions (37 °C, 5% CO₂) |
| Recommended Buffer System | Sodium bicarbonate/CO₂ buffering system; HEPES-buffered options available |
| Application Notes / Precautions | Pre-warm to 37 °C before use; supplement with serum and antibiotics as needed; use aseptic technique |
| Batch-to-Batch Consistency | Osmolality within ±5% of reference; cell growth rate within ±15% of reference lot |
For research use only, not for clinical use.
|
There is no product in your cart. |
Copyright © 2026 Alta DiagnoTech. All rights reserved.
