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| Product Name | FspI Rapid Restriction Endonuclease (10 U/uL) |
| Catalog No. | IVDRM-0418 |
| Description | Cutting the palindromic site 5 prime TGC^GCA 3 prime to give blunt ends, this rapid recombinant endonuclease acquires and processes DNA fragments for molecular cloning and finishes digestion of plasmid DNA, PCR products and genomic DNA in 5 to 15 minutes. Supplied at 10 U/uL, it runs in the unified rapid series buffer at 37°C and is heat inactivated at 80°C for 20 minutes. CpG methylation fully overlapping the site blocks cleavage, and the isoschizomers Acc16I, AviII, MstI and NsbI share the specificity. |
| Intended Use | DNA fragment acquisition and processing for molecular cloning with blunt ended digestion |
| Principle / Technology | Site specific blunt cleavage at 5 prime TGC^GCA 3 prime within 5 to 15 minutes at 37°C |
| Specificity | CpG methylation fully overlapping the site blocks cleavage; isoschizomers Acc16I, AviII, MstI and NsbI |
| Reaction Conditions / Protocol | Digest in 1x rapid restriction buffer at 37°C for 5 to 15 minutes; heat inactivate at 80°C for 20 minutes |
| Storage Conditions | -25 to -15°C |
| Shelf Life | 2 years |
| Package Specifications | 50 T |
| Key Features | Blunt end output; single shared buffer across the rapid series |
| Concentration | 10 U/uL |
| Readouts / Downstream Assays | Gel electrophoresis of digested fragments |
For in vitro diagnostic use.
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