| Product Name | Free Hemoglobin (FHb) Content Assay Reagent (Visible Spectrophotometer) |
| Catalog No. | PTR-HMM-0041 |
| Description | Free hemoglobin (FHb) refers to hemoglobin molecules in plasma that are not bound to red blood cell membranes. When hemolysis occurs, red blood cells rupture, releasing hemoglobin and increasing the levels of free hemoglobin in plasma. Changes in its levels are important in cellular immunity, trauma treatment, and the diagnosis of hemolytic diseases. |
| Application | The ferrous hemoglobin in hemoglobin has catalytic activity similar to that of peroxidase, enabling it to catalyze the oxidation of 4-aminoantipyrine and aniline analogues by hydrogen peroxide to form a purple compound. The product exhibits a characteristic absorption peak at 546 nm, and the concentration of free hemoglobin can be quantitatively determined by measuring changes in absorbance. |
| Applicable Instruments | Visible Spectrophotometer |
| Number of Testable Samples | 48 Samples |
| Matching | 1 mL glass cuvette (d=10 mm) |
| Detection Time | 2 h (48 Samples) |
| Detection Method | 4-Aminoantipyrine |
| Spectral Parameters | 546 nm |
| Signal Response | Incremental |
| Notes | Avoid hemolysis during sampling and plasma separation; if the A measurement is greater than 1.0, it is recommended to dilute the sample with distilled water before testing; If the A measurement is less than 0.02, it is recommended to increase the sample volume during sample preparation, re-extract, and then perform the measurement, making corresponding adjustments during calculation. |
For research use only, not for clinical use.
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