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| Product Name | Biuret Method Protein Content Assay Reagent (Visible Spectrophotometer) |
| Catalog No. | PTR-HMM-0033 |
| Description | The bicinchoninic acid (BCA) protein assay is a commonly used method for colorimetric detection and total protein quantification, characterized by its simplicity, good reproducibility, good linearity, and stable reagents. |
| Application | Proteins can form a purple complex with the biuret reagent in strongly alkaline solutions, with the product exhibiting a characteristic absorption peak at 540 nm. The protein content can be quantitatively detected by measuring changes in absorbance. |
| Applicable Instruments | Visible Spectrophotometer |
| Number of Testable Samples | 50 Samples |
| Matching | 1 mL glass cuvette (d=10 mm) |
| Detection Time | 3 h (50 Samples) |
| Detection Method | Biuret |
| Spectral Parameters | 540 nm |
| Signal Response | Incremental |
| Standard | BSA |
| Reference Standards | y=0.0425x-0.001 (R2=0.9992) |
| Standard Linear Range | 0.5-10 mg/mL |
| Detection Limit | 0.5 mg/mL |
| Notes | The protein concentration of the sample to be tested must be within the range of 0.5–10 mg/mL. For formal testing, 1–2 samples with different concentrations should be selected for preliminary testing to ensure that the protein concentration is within this range. If the concentration is below 0.5 mg/mL, this method cannot be used for testing. If the concentration is above 10 mg/mL or the absorbance value is greater than 0.4, the sample should be appropriately diluted with Extract Solution before testing, and the calculation should be adjusted accordingly. |
For research use only, not for clinical use.
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